TIN: Week 3

Question 1

What stain is used to visualize DNA and RNA?

Answer 1

Stain: Aniline dyes that stain purple or dark blue.
Mechanism: These work by ionic interaction.

Question 2

What are aniline dyes?

Answer 2

Synthetic dyes made from analyn that can be obtained from cold tar.

Question 3

Describe Luxol Fast Blue dyes:

Answer 3

Stains: Myelin Sheath in blue
Mechanism: ionic interaction between the dye (anion) and myelin lipoproteins (cation) which initiates binding
Indication: study of myelinated nerve tracs and changes or loss of myelin in neurodegenerative disease

Question 4

Describe Luxol Fast Blue dyes:

Answer 4

Stains: Myelin Sheath in blue
Mechanism: ionic interaction between the dye (anion) and myelin lipoproteins (cation) which initiates binding
Indication: study of myelinated nerve tracs and changes or loss of myelin in neurodegenerative disease

Question 5

What is an example of a metal impregnation stain?

Answer 5

Golgi Stain- not a true stain but a method of precipitation
Stains: 1-10% of neurons dark brown or black
Mechanism: silver precipitate produces insoluble black reaction to product
Indications: study of neuronal morphology (such as dendritic spines)

Question 6

What are the 5 types of antibodies and which is most often an antibody reagent?

Answer 6

1) IgG
2) IgM
3) IgA
4) IgD
5) IgE

Most antibody reagents are IgG