Ti Plasmid Flashcards
What does Ti Plasmid do?
It integrates a segment of its own DNA, also known as TDNA, into the chromosomal DNA of its host plant cells.
What is used to produce transgenic plants?
Ti Plasmid
What is the first step?
Isolating the Ti Plasmid from the bacterium (Agrobacterium tumefaciens)
What is the segment of the plasmid that ingrates into the genome of host cells?
TDNA
What is the second step?
The foreign gene of interest is inserted into the TDNA.
What happens by electroporation?
Recombinant plasmids can be introduced into cultured plant cells.
What happens if the plasmids are not introduced into cultured plant cells?
Plasmids can be returned to agrobacterium.
The agrobacterium is applied as a liquid suspension to what?
The leaves of susceptible plants (which infects them)
What happens once the plasmid is taken into a plant cell?
The TDNA integrates into the cells chromosomal DNA.
What happens as a result of the TDNA being integrated into the cells chromosomal DNA?
The transformed cells that carry the transgene of interest can regenerate complete plants.
What will the plants do after the process is over?
Exhibit the new trait confirmed by the transgene.
