Thrombin-like enzymes

Question 1

Structure of the enzyme

Answer 1

• SVTLE ranges from 26-67 kDa
  
  • 232-236 amino acid residues
  • And often glycosylated
  • Typical beta fold similar to trypsin = 2 beta-barrels and each contains 6 beta strands with insertion of short beta strands and 3 alpha helices.
    His57,Asp102 and Ser195 are conserved in most of the SVTLEs

Question 2

moa

Answer 2

• During envenomation, the enzymes shown prominently : fibrinogenolysis, fibrinolysis, platelet aggregation, thrombosis, activation of coagulation factor etc.
  
  • Acts directly on fibrinogen to induce a clot by producing insoluble fibrin from fibrinogen
  • The clot is weak so it is is being removed = leading to defibrinogenated plasma
  • Does not activate factor XIII and thus no cross linking = frail clots
    Remove fibrinogen in ur body = thus ,blood becomes unclottable = increase of risk of bleeding out

Question 3

toxin to therapeutics - thrombin like enzymes

Answer 3

• Used as defibrinogentating agent in patients suffering from thrombosis and myopcardial infarction, peripheral vascular diseases and acute ischemia.
  
  • Fibrin sealant in some medical conditions
    Dissolve calcium oxide stones