Thermodynamics Flashcards
1
Q
[PL]
A
Protein-ligand complex conc.
2
Q
[L]
A
Ligand conc.
3
Q
[P]
A
Protein conc.
4
Q
Dissociation constant
A
Kd
5
Q
Association constant
A
Ka
6
Q
Kd units
A
molddm-3
7
Q
Ka units
A
Mol-1dm3
8
Q
Relationship between Kd and Ka
A
Kd=1/Ka
9
Q
B
A
Fractional occupancy = [PL]/([P]+[PL])
10
Q
Langmuir Isotherm (B as a function of [L])
A
B = [L]/(Kd+[L])
11
Q
Linearized Langmuir Isotherm
A
ln(B/(1-B)) = ln[L] - lnKd
12
Q
Gradient of a langmuir isotherm
A
1
13
Q
Quick dertemination of Kd
A
On a graph of x = [L] and y = B
- Kd = [L]1/2
14
Q
Hill Isotherm (B as a function of [L])
A
B = [L]^n/(Kd + [L]^n)
15
Q
Linearized Hill isotherm
A
ln(B/(1-B)) = nln[L] - lnKd
