The Pyruvate Dehydrogenase System Flashcards

1
Q

Anaplerotic

A

(Filling up) reactions that replenish the TCA cycle intermediates

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2
Q

Major anaplerotic reaction

A

Pyruvate Carboxylase is a major anaplerotic reaction in mammalian tissues
Pyruvate+CO2+ATP+H2O—->Oxaloacetate+ADP+Pi

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3
Q

Pyruvate dehydrogenase

A

Links glycolysis to the TCA cycle

Pyruvate—>Acetyl-CoA
Gives off H2 and CO2

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4
Q

Entry of pyruvate into the mitochondria

A

Pyruvate translocase transports pyruvate into the mitochondria in symport with H+

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5
Q

Reactions of the PDH complex

A
Multi enzyme with 3 type subunits
Pyruvate dehydrogenase (E1)
Dihydrolipoyl transacetlyase (E2)
Dihydrolipoyl dehydrogenase (E3)
E2&E3 are inhibited by NADH, acetyl CoA and CO2
Activated by pyruvate, NAD+ and HSCoA
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6
Q

Activation and inhibition of the PDH

A

Pyruvate dehydrogenase kinase phosphorylates the PDH inactivating it (uses ATP)

Pyruvate dehydrogenase phosphatase cleaves the phosphate from the inactive form activating it. Uses water and produces Pi

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