The Microscope Flashcards
It is an indispensable instrument in the study of cells.
Microscope
On/off switch and intensity knob.
Light Source
Controls the intensity of the light coming from the bulb
Rheostat knob
Device found above the light source that converges the light beams into a point so that they can enter the specimen.
Condenser
raises and lowers the condenser, so that the point of light entering the specimen can be varied.
Knob
works like the iris of your eye, a muscle that controls the amount of light entering the eye. It is found within the condenser and a lever is provided so the amount of light entering the specimen from the condenser can be varied.
Iris Diaphragm Lever/Control
where the slide is placed and has a central opening to allow light from the condenser to pass through the specimen.
Stage
pulling the slide clip towards you allows for the placement of the slide, flat on the stage.
Slide clip
this is a double knob found below the stage that allows for movement of the slide.
Stage adjustment knobs
What does the lower knob do?
Y axis/ Vertical axis
What does upper knob do?
X axis/ horizontal axis
- after the light passes through the specimen it then is picked up by the ______ that are housed within the revolving nosepiece.
Objectives
allows for the movement of different power objectives into position over the specimen.
Revolving nosepiece
magnify the image that is received.
Objectives
There are four objectives housed on the revolving nosepiece. List the magnification of each and its designation.
Scanning 4x, LPO 10x, HPO 40x, OIO 100x
light from the objective after being magnified travels through the ______ and is directed to the ocular by being reflected off a mirror.
Body Tube
The ______ also have the ability to magnify the image by 10X.
Ocular
Adjust the oculars by sliding them either closer together or further apart until you see one field of view when using both eyes.
Intrapupillary Distance Adjustment:
Use the ______ (gives approximate focus)
Coarse Adjustment Knob
Any fine-tuning of the focus is accomplished with the ______ (Gives exact focus) while looking through the oculars.
Fine Adjustment Knob
This microscope is ______ meaning that when you switch to a different objective the image should remain in perfect focus.
parfocal
Remember the image is magnified _____, once by the objective and second by the ocular.
twice
a numerical measure of the resolution of the lens. The smallest distance between two objects that the microscope is able to distinguish as two distinct points.
Resolving Power
Formula of Resolving Power
RP = wavelength of light / 2NA
the distance between two troughs of the light wave, we will be using the average wavelength of visible light or 0.55 m
Wavelength of light
It is the area within which objects are visible with microscope oculars and objectives of various magnifying powers.
Microscopic field
It is an optical device, providing good depth effect, for use with both eyes, consisting of two small telescopes fitted together side by side, each telescope having two prisms between the eyepiece and objective for erecting the image.
Binocular
Calculate the resolving power for each objective.
RP of 4X objective
0.55/2(0.10)=2.75um
