The Microscope

Question 1

It is an indispensable instrument in the study of cells.

Answer 1

Microscope

Question 2

On/off switch and intensity knob.

Answer 2

Light Source

Question 3

Controls the intensity of the light coming from the bulb

Answer 3

Rheostat knob

Question 4

Device found above the light source that converges the light beams into a point so that they can enter the specimen.

Answer 4

Condenser

Question 5

raises and lowers the condenser, so that the point of light entering the specimen can be varied.

Answer 5

Knob

Question 6

works like the iris of your eye, a muscle that controls the amount of light entering the eye. It is found within the condenser and a lever is provided so the amount of light entering the specimen from the condenser can be varied.

Answer 6

Iris Diaphragm Lever/Control

Question 7

where the slide is placed and has a central opening to allow light from the condenser to pass through the specimen.

Answer 7

Stage

Question 8

pulling the slide clip towards you allows for the placement of the slide, flat on the stage.

Answer 8

Slide clip

Question 9

this is a double knob found below the stage that allows for movement of the slide.

Answer 9

Stage adjustment knobs

Question 10

What does the lower knob do?

Answer 10

Y axis/ Vertical axis

Question 11

What does upper knob do?

Answer 11

X axis/ horizontal axis

Question 12

• after the light passes through the specimen it then is picked up by the ______ that are housed within the revolving nosepiece.

Answer 12

Objectives

Question 13

allows for the movement of different power objectives into position over the specimen.

Answer 13

Revolving nosepiece

Question 14

magnify the image that is received.

Answer 14

Objectives

Question 15

There are four objectives housed on the revolving nosepiece. List the magnification of each and its designation.

Answer 15

Scanning 4x, LPO 10x, HPO 40x, OIO 100x

Question 16

light from the objective after being magnified travels through the ______ and is directed to the ocular by being reflected off a mirror.

Answer 16

Body Tube

Question 17

The ______ also have the ability to magnify the image by 10X.

Answer 17

Ocular

Question 18

Adjust the oculars by sliding them either closer together or further apart until you see one field of view when using both eyes.

Answer 18

Intrapupillary Distance Adjustment:

Question 19

Use the ______ (gives approximate focus)

Answer 19

Coarse Adjustment Knob

Question 20

Any fine-tuning of the focus is accomplished with the ______ (Gives exact focus) while looking through the oculars.

Answer 20

Fine Adjustment Knob

Question 21

This microscope is ______ meaning that when you switch to a different objective the image should remain in perfect focus.

Answer 21

parfocal

Question 22

Remember the image is magnified _____, once by the objective and second by the ocular.

Answer 22

twice

Question 23

a numerical measure of the resolution of the lens. The smallest distance between two objects that the microscope is able to distinguish as two distinct points.

Answer 23

Resolving Power

Question 24

Formula of Resolving Power

Answer 24

RP = wavelength of light / 2NA

Question 25

the distance between two troughs of the light wave, we will be using the average wavelength of visible light or 0.55 m

Answer 25

Wavelength of light

Question 26

It is the area within which objects are visible with microscope oculars and objectives of various magnifying powers.

Answer 26

Microscopic field

Question 27

It is an optical device, providing good depth effect, for use with both eyes, consisting of two small telescopes fitted together side by side, each telescope having two prisms between the eyepiece and objective for erecting the image.

Answer 27

Binocular

Question 28

Calculate the resolving power for each objective.

RP of 4X objective

Answer 28

0.55/2(0.10)=2.75um