The Microbial World Flashcards

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1
Q

What is Microbiology?

A

The study of microbes, or forms of life too small to be seen with the naked eye.

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2
Q

What are the characteristics of life?

A

Metabolism, growth, reproduction, genetic variation/evolution, response/adaptation to external environment, and maintaining homeostasis.

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3
Q

What is the definition of life?

A

“A self-organizing, self replicating, non-equilibrium system”.

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4
Q

What are the 4 macromolecules needed for life in general?

A

Polypeptides, nucleic acids, lipids, and polysaccharides.

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5
Q

What are the functions of Polypeptides? Dry Weight of cell (%)?

A

Enzymes catalyze the vast majority of biochemical reactions in the cell. Other proteins are structural components of cells. 50-55%.

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6
Q

What are the functions of DNA Nucleic acids? Dry Weight of cell (%)?

A

Informational: DNA provides the instructions for assembly and reproductions of the cell. 2-5%.

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7
Q

What are the functions of RNA Nucleic acids? Dry Weight of cell (%)?

A

Many functions, most of which are involved in the production of polypeptides. Some serve structural or catalytic functions. 15-20%.

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8
Q

What are the functions of Lipids? Dry Weight of cell (%)?

A

Structural: Make up cellular membranes that form physical boundary between the inside of the cell and surroundings and membranes of internal organelles. 10%.

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9
Q

What are the functions of Polysaccharides? Dry Weight of cell (%)?

A

Structural (cellulose and chitin) and energy storage (glycogen and starch). 6-7%

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10
Q

What are the subunits of Polypeptides, DNA/RNA Nucleic acids, Lipids, and Polysaccharides respectively?

A

Amino acids, deoxyribonucleotides, ribonucleotides, “diverse structures”, sugars.

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11
Q

DNA sequence are how we can break life into 3 large groups known as ____ which are ____, ____, ____.

A

Domains; prokaryotes, eukaryotes, and archea.

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12
Q

What are viruses?

A

a metabolically inert, infectious agent that replicates only within the cells of living hosts.

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13
Q

What are some reasons microbes are important?

A

They’re fast, cheap and easy to grow. They can produce enzymes and other molecules for industrial/medical uses. Most have a small number of genes, making them easier to study, and genetic manipulation of single-celled bacteria is usually much easier to study than multicellular eukarya.

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14
Q

Explain the Endosymbiotic theory.

A

Primitive prokaryotic microbes ingested other microbes, starting a symbiotic relationship, forming the first basic eukaryotes.

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15
Q

What is the basic idea of how microbial life arose on earth?

A

Early conditions formed RNA and micelles, which came together into a primitive cell using RNA for storing genetic information and coding. Primitive cells eventually changed from using RNA to DNA instead for storing their genetic information.

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16
Q

Bacteria and humans today use double-stranded DNA for storage of genetic information rather than RNA. Why the Change?

A

dsDNA contains a “backup copy” of genetic information and is more stable than RNA.

17
Q

The first microbes were observed from ____ to ____ by ____________.

A

1623-`673; Anton Van Leeuwenhoek.

18
Q

In 1665, ____ ____ came up with the Cell Theory, which states ….

A

All living things are composed of cells.

19
Q

What is the spontaneous generation hypothesis?

A

the hypothesis that life arises from nonliving matter; a “vital force” is necessary for life. Joseph Needham (1745)

20
Q

What is the biogenesis hypothesis?

A

the hypothesis that living cells arise only from preexisting living cells. Lazzaro Spallanzani (1765).

21
Q

What did Louis Pasteur’s experiment succeed in doing?

A

Louis Pasteur’s experiment disproved the spontaneous generation theory in the late 1800s.

22
Q

What were some of Robert Koch’s accomplishments?

A

Koch determined Bacillus anthracis and Mycobacterium tuberculosis were the causes of anthrax and tuberculosis (respectively). His work with anthrax helped sheep herders and cattle ranchers avoid costly animal losses.

23
Q

What is the Germ theory? Who discovered it?

A

Discovered by Koch, the Germ theory states that many diseases are caused by the presence and actions of specific micro-organisms within the body.

24
Q

Reduction of deaths from microbes and diseases as a result of the prevention of infection include:

A

use of antiseptics, sanitation improvements, pasteurization, personal hygiene improvements, and vaccination.