The Krebs Cycle Flashcards
Where does the Krebs cycle take place?
The mitochondrial matrix
Name the products of one turn of the Krebs cycle
Three NADH
One FADH2
One GTP
Two CO2
Name the products of the Krebs cycle from one glucose
Six NADH
Two FADH2
Two GTP
Four CO2
How many carbons are there in Oxaloacetate?
Four
Describe the production of citrate from oxaloacetate
Citrate synthase converts oxaloacetate to citrate using one molecule of Acetyl-CoA
Name the enzyme used in the isomerisation of citrate
Aconitase
What type of reaction is the conversion of isocitrate to alpha-ketoglutarate
Dehydrogenation
Oxidation
How many cartons has alpha-ketoglutarate
Five
What other byproducts of the enzyme isocitrate dehydrogenase?
NADH
CO2
H+
Name of the enzyme that converts isocitrate into alpha-ketoglutarate
Isocitrate dehydrogenase
Name the complex responsible for the conversion of alpha-ketoglutarate to succinyl-CoA
Alpha-ketoglutarate dehydrogenase complex
What are the byproducts of the conversion of alpha-ketoglutarate to succinyl-CoA?
NADH
CO2
H+
How many carbons are there in succinyl-CoA
Four
What is added to alpha-ketoglutarate by the alpha-ketoglutarate dehydrogenase complex?
CoA
What is the enzyme responsible for the conversion of succinyl-CoA to succinate
Succinyl-CoA synthetase
How is the CoA removed from succinate and which byproduct does this result in?
CoA Is displaced by phosphate molecule.
The phosphate molecule is transferred to GDP forming GTP
Name the byproducts of the reaction catalysed by Succinyl-CoA synthetase
CoA
GTP
Where might you find an isoform of succinyl-CoA synthetase that produces ATP instead of GTP?
Skeletal and cardial muscle
What is fumarate produced from and which type of reaction is this?
Succinate
Dehydrogenation (Oxidation)
Which enzyme catalyses the production of fumarate and what are its byproducts?
Succinate dehydrogenase
FADH2
Name the four carbon sugars involved in the TCA cycle
Succinate
Fumarate
Malate
Oxaloacetate
Name the six carbon sugars involved in the TCA cycle
Citrate and isocitrate
Which enzymes act on fumarate and what does it produce?
Fumarase
Malate
What type of reaction does Fumerase catalyse?
Hydrolysis
In Addition of water breaking a double bond
Which enzyme produces oxaloacetate from Malate?
Malate dehydrogenase
Name all of the dehydrogenase enzymes involved in the TCA cycle
Isocitrate dehydrogenase
Alpha ketoglutarate dehydrogenase complex
Succinate dehydrogenase
Malate dehydrogenase
Name the three enzymes to produce NADH in thes TCA cycle
Isocitrate dehydrogenase
Alpha ketoglutarate dehydrogenase complex
Malate dehydrogenase
Which is the only enzyme is not in the mitochondrial matrix and where is it located?
Succinate dehydrogenase; Integral to the inner mitochondrial membrane
