The Cell Surface Flashcards

1
Q

what is the structure of the cell membrane?

A
  • Composed of lipids ( mainly phospholipids) and proteins
  • There are two opposing sheets of lipids into which proteins are inserted (called a bilayer)
  • Hydrophobic tails face out towards fluids
  • Fatty acid chains determine fluidity of membrane
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2
Q

what is the structure of a phospholipid?

A

has a hydrophobic tail and a hydrophilic head

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3
Q

what does amphipathic mean?

A

they have a polar and a hydrophobic part

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4
Q

what is the function of a cell membrane?

A

barrier- between the cells/cell organelles and their environment, controlling which substances enter and leave.
compartmentalisation – maintain different internal environments
Site of chemical reactions – inner membrane of mitochondria (cristae) contains enzymes needed for cellular respiration.
Membranes also protect vital cell components e.g. DNA.
cell recognition- e.g. cells of the immune system. (Glycolipids)
cell communication - (cell signalling (Glycoproteins)

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5
Q

what are the 4 major phospholipids in the mammalian PM?

A
  • phosphatidylcholine
  • phosphatidylserine
  • phosphatidylethanolamine
  • sphingomyelin
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6
Q

how are all the phospholipids able to link together?

A

They all have similar structure so are able to link together
Only head group is different (EXCEPT
Sphingomyelin)

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7
Q

what are intracellular signal transduction lipids?

A

They are a minor proportion of the phospholipid content of intracellular membranes.
Derived from lipids residing in the PM
Bind specifically to conserved regions found within many different proteins and once bound, induce conformational and/ or localisation and activity changes within these proteins

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8
Q

what are the features of intracellular signal transduction lipids?

A

Rapidly generated/ destroyed by enzymes in response to a specific signal (to prevent continuous signals)
They are spatially and temporally (at a specific space at a specific time) generated giving a highly specific signal.

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9
Q

what are 4 examples of intracellular signal transduction lipids?

A

▪ Phosphatidylinositol
▪ Diacylglycerol
▪ Ceramide
▪ Sphingosine-1-phosphate

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10
Q

what are glycolipids?

A

located on non-cytosolic half of membrane
glycosylated in Golgi apparatus
Important for recognition and cell attachment

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11
Q

what is the structure of cholesterol?

A
  • 4-ringed ridged planar structure
  • Polar head group interacts with the hydrophilic head
  • hydrophobic hydrocarbon tail
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12
Q

what is the role of cholesterol in the PM?

A

Inserts between membrane phospholipids
This tightens packing in the bilayer/ membrane rigidity and decreases membrane permeability to small molecules
Regulates cell membrane fluidity
At 37C cholesterol makes the membrane less fluid – stabilises interactions between neighbouring phospholipids

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13
Q

what does cholesterol do to proteins?

A

Cholesterol also stabilises protein groups which makes communication easier

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14
Q

what does the fluidity of the PM allow it to do?

A

Allow signalling lipids and membrane proteins to rapidly diffuse in the lateral plane and interact with one another
Fluidity is important in cell division- ensures membranes are equally shared between daughter cells following cell division
Allows membranes to fuse with other membranes e.g. in exo/endocytosis

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15
Q

what are transmembrane proteins and why are they integral?

A

proteins that span the entire lipid bilayer
integral bc they go through both layers

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16
Q

what are transmembrane domains and what do they consist of?

A

transmembrane domains - regions in the membrane of integral proteins

mainly contain hydrophobic amino acid side which are in contact with the phospholipid tails

the proteins are usually organised in alpha helical structures

17
Q

what are other examples of transmembrane proteins?

A

□ Beta barrel protein
□ Lipid linked- proteins that are post translationally modified (lipid is added) allowing it to anchor to the PM
□ Peripheral membrane proteins- proteins that interact with other integral proteins

18
Q

what are transmembrane protein functions? (SIGRATE)

A
  • Signal transduction molecules: pass on and amplify signals.
  • Intracellular joining – joining of two cells
  • Glycoproteins – allows for cell-cell recognition
  • Receptors – ligands bind to a receptor to allow for the transduction of a signal into the cell
  • Anchors – integrins, link the extra cellular matrix with actin of the cytoskeleton (allows cellular motility)
  • Transporters – channel and carrier proteins
  • Enzymes
19
Q

What are the 2 different ways of transport across the PM?

A

Active- needs ATP
Passive- doesn’t need ATP

20
Q

What are the 2 examples of passive transport?

A

Simple diffusion
facilitated diffusion

21
Q

what is simple diffusion?

A

It is just driven by the concentration gradient.
- No membrane proteins involved
- Driven by concentration gradients
- Membranes are highly impermeable to ions

22
Q

what are the molecules involved in simple diffusion?

A

○ Hydrophobic molecules can freely diffuse across the membrane down their concentration gradients e.g. O2, CO2, N2 and benzene
○ Small uncharged polar molecules can also diffuse across the membrane (although this is to a lesser extent than hydrophobic molecules) e.g. H2O, urea, glycerol
○ Large uncharged polar molecules (e.g. glucose and sucrose) are too large to diffuse across the membrane
○ All ions are too polar to diffuse across the membrane

23
Q

what is facilitated diffusion?

A

Membrane proteins are involved
They transport inorganic ions/ small molecules across the membrane passively (downhill) along their concentration/ electrochemical gradient
It is also driven down a concentration gradient.

24
Q

what are the 2 classes of channels involved in facilitated diffusion?

A

1) Channels - discriminates mainly on size and charge
2) Uniporter carrier proteins - involves a binding site for solutes

25
Q

what is the structure of the channels?

A

○ Most are non-directional ion channels
○ Hydrophilic pores are formed through the membrane (Hydrophilic surfaces)
○ Show some selectivity on molecules that pass through based on size and charge
○ Fast

26
Q

what are uniporter carrier proteins and give an example?

A

○ Carries one type of molecule
○ Highly selective (as molecule needs to bind to a specifically shaped site)
○ Binding of the molecule to a specific site causes a small conformational change to the carrier protein resulting in movement of molecule from inside to outside or vice versa
○ Carrier proteins are required for almost all small organic molecules
○ Relatively slow
E.g. glut 2 in gut epithelium

27
Q

define an electrochemical gradient

A

Defined as.. the net force driving a charged solute across a membrane.

28
Q

define an electrochemical gradient

A

Defined as.. the net force driving a charged solute across a membrane.

29
Q

what is the electrochemical gradient composed of?

A

It is composed of a concentration gradient and the voltage across the membrane.
An electrochemical gradient combines the concentration gradient and membrane potential
The force driving a charged solute (e.g Na+ ions) across a membrane is the concentration gradient and the membrane potential

30
Q

what are the reasons to maintain an electrochemical gradient?

A

○ It drives transport across the membrane
○ Maintains the osmotic balance - without active transport to maintain EC gradient, ions would flow down their gradients through leak channels so the osmotic balance is disturbed which results in cell death

31
Q

what is the difference between primary and secondary active transport?

A

primary active transport: the energy is derived directly from the breakdown of ATP.

secondary active transport: the energy is derived secondarily from energy that has been stored in the form of ionic concentration differences between the two sides of a membrane

32
Q

what is active transport?

A

Moves solutes against their chemical gradient by expanding energy (primary active transport)
requires ATP

33
Q

Explain the Na+K+ ATPase example (Primary active transport)

A

Na+ and K+ electrochemical gradient:
· In the absence of Na+ / K+ ATPase ions would flow down their gradients, disturbing osmotic balance and preventing ‘secondary active transport’
· Found in the plasma membrane of all animal cells
· There is a higher concentration of K+ inside the cell than outside and a higher concentration of Na+ outside the cell than inside
· 3 Na+ pumped out of the cell for every 2 K+ moved in.
· 3 Na+ bind to the catalytic subunit, this activates ATPase
· ATP undergoes hydrolysis resulting in the phosphorylation of a subunit of ATPase. ATPase undergoes a conformation change which causes the release of 3 Na+ out of the cell
· 2 K+ then bind extracellularly
· The K+ binding causes the subunit to undergo dephosphorylation so the pump changes conformation to its original and 2 K+ moves into the cell
· If one step is blocked then pump is halted
· whole cycle takes 10 milliseconds

34
Q

what are coupled transporters?

A

Couple the transport of one solute with its conc gradient to another against its gradient
Do not depend directly on the hydrolysis of ATP (secondary active transport)

35
Q

what is the difference between symport and antiport transporters?

A

· symport – both solutes move in same direction
· antiport – solutes move in opposite direction

36
Q

explain the Na+/ glucose symporter in the gut epithelia (secondary AT)

A
  • Higher Na+ concentration & lower glucose concentration in the Gut (extracellular)
  • The binding of glucose is dependent on the binding of Na+ (co-operative binding)
  • As there is a higher concentration of Na+ outside the cell glucose more likely to bind to the symporter when its in its initial state – allows for binding of extracellular molecules.
  • As a result there is an overall net flow of glucose (against its concentration gradient) and Na+ (along its concentration gradient) into cell.
  • One of the reasons you get a build-up of Na+ in cells is because it is being used in symporters to transfer other molecules

Binding of Na+ and glucose is cooperative i.e binding of glucose is dependant on Na+
Bc Na+ conc is much higher outside the cell, glucose is more likely to bind in state A than state B
Over all result is that Na+ and glucose enter the cell more than leave it therefore NET FLOW is into the cell

37
Q

explain the Na+/Ca2+ antiporter (secondary active transport)

A
  • Important in cardiac muscle
  • Cardiac muscle contraction is triggered by rise in intracellular Ca2+ concentration
  • There is a higher concentration of Na+ and Ca2+ outside the cell than inside the cell
  • Therefore, the antiporter moves 3 Na+ in to the cell (along its concentration gradient) for every 1 Ca2+ out (against its concentration gradient)
  • The antiporter therefore rapidly reduces intracellular Ca2+ concentration which reduces the strength of cardiac muscle contraction i.e. causes cardiac muscle relaxation