Tetrahydrofolate, Vitamin B12, and S-Adenosylmethionine

Question 1

Folate deficiency before and during pregnancy is associated with neural tube defects such as…

Answer 1

Spina Bifida

Question 2

An inherited mutation in the proton coupled folate transporter (PCFT; gene SCL46A1) causes a functional folate deficiency despite adequate folate in the diet. With what disease is this associated?

Answer 2

Hereditary folate malabsorption

Question 3

Where does folate go when you have too much in the body?

Answer 3

It’s stored in the bile ducts

Question 4

What is the main common result of folate deficiency?

Answer 4

Megaloblastic anemia: large red blood cells.

Question 5

Dihydrofolate reductase carries out how many reactions to reach tetrahydrofolate?

Answer 5

Two, both add 2 H each time.

Question 6

What drugs target dihydrofolate reductase in humans?

Answer 6

Methotrexate (for use in cancer and rheumatoid arthritis)

Question 7

Between 5-Formyl THF, 5-Forminmino THF, and 5-Methyl THF, which is most oxidized, which is most reduced and which is the intermediate?

Answer 7

5-Formyl THF: Most ox.
5-Forminmino THF: intermediate
5-Methyl THF: Most reduced

Question 8

In tryptophans processing, it creates what important molecule for folate mechanisms?

Answer 8

Formate

Question 9

Which step in 5- methyl THF synthesis is irreversible?

Answer 9

5,10 Methylene THF –> 5-Methyl THF

Question 10

When histidine donates a C to THF, what is the intermediate and product?

Answer 10

Intermediate = 5-Formino TH (FIGLU)
Product = 5,10 Methenyl THF

Question 11

Serine is the most important C donor to THF because it contribute the most C’s out of any other donors (T/F)

Answer 11

T