Tests spécimens d'urine Flashcards

1
Q

Staphylococcus et micrococcus (6)

-les tests fait à tout

A
  1. gram (cocci +)
  2. catalase (+)
  3. coagulase libre
  4. novobiocine
  5. KB
  6. rien sur MacConkey
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2
Q

Micrococcus (7)

A
  1. gram (cocci +)
  2. catalase +
  3. coagulase -
  4. novobiocine sensible >= 16
    * 5. furazolidone résistant < 14
    * 6. microdase +
  5. KB

*5 et 6 faire seulement s’il y a un pigment jaune

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3
Q

Staph aureus (3)

A
  1. coagulase +
  2. novo : ignore
  3. KB
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4
Q

Staph saprophyticus (3)

A
  1. coagulase -
  2. novo résistant < 16
  3. KB
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5
Q

Staph à coagulase négative(5)

A
  1. coagulase -
  2. novo sensible > 16
  3. fura sensible >15
  4. microdase -
  5. KB
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6
Q

KB, si staph sensible pénicilline

A

on fait le bêta-lactamase

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7
Q

incubation pour les urines

A

24h

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8
Q

urine, technique qualitative ou quantitative

A

quantifier ex compter 65 colonies = 65 000 bact/mL

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9
Q

Hémolyse

  1. vert
  2. clair
  3. aucun
A
  1. alpha
  2. bêta
  3. gamma
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10
Q

Streptococcus bêta (4)

A
  1. gram cocci+
  2. catalase -
    * 3. prolex : A, B, C, D, F ou G
  3. pas KB = ce germe est habituellement sensible à la pénicilline
  • Strep throat = groupe A
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11
Q

Strep alpha (6)

strep pneumo

A
  1. gram cocci +
  2. catalase -
  3. Bile esculine -
  4. NaCl -
    * 5. Optochin sensible <14
  5. lyse de la bile si optochin est entre 10 et 14 = soluble
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12
Q

Strep non-hémo (5)

ou Enterococcus faecalis

A
  1. gram cocci +
    * 2. catalase -
  2. bile +
  3. NaCl -
    * 5. PYR

*Enterococcus faecalis = catalase - (faible/lent) et PYR +, aussi bêta lactimase - / KB

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13
Q

Strep du groupe viridans (5)

A

1.gram cocci +
2. catalase -
3. optochin résistant < 10
4. lyse de la bile insoluble
5. bile + NaCl - (groupe D non-enterocoque)
bile - NaCl - (bêta hémo)

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14
Q

Strep/Entero tu fais KB

A

entero faecalis et pneumo

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15
Q

Enterobacteriaceae (4)

A
  1. gram bacille -
  2. pousse sur MacConkey
  3. regarde si fermente le lactose
  4. regarde ‘‘texture’’ exemple rugueux
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16
Q

Lactose + et rugueux (4)

A
  1. gram bacille -
    * 2. spot indole
  2. API 20E
  3. KB

*faire l’API si spot indole -, et si spot indole + = E coli

17
Q

Lactose + et muqueux (4)

A
  1. gram bacille -
  2. API 20E
  3. Urée
  4. KB
18
Q

Lactose + et Lisse (3)

A
  1. gram
  2. API 20E
  3. KB
19
Q

Lactose -

A
  1. gram bacille -
    * 2. oxydase -
  2. API 20E
  3. KB

*si + faire test pour pseudo

20
Q

Pseudo (8)

A
  1. gram bacille -
  2. oxydase +
  3. mobilité +
  4. OF glucose x 2 avec et sans huile, oxydatif : jaune sans huile et vert avec l’huile
  5. pseudo p = pigment
  6. pseudo f = fluorescent
  7. API
  8. KB

*vise pas les bouchons