TESTS Flashcards
Gmelin
test for bile pigments
Lugol’s iodine + alcoholic solution of zinc acetate
Schlesinger
Schlesinger
test for bile pigments
Schlesinger (+) result
greenish fluorescence
Gmelin (+) result
play of colors
tests for hemoglobin
Benzidine
Guaiac
Ortho-Toluidine
(+) results for tests for hemoglobin
Benzidine = green-blue Guaiac = blue Ortho-Toluidine = blue
Sulkowitch is a test for
Calcium
(+) Sulkowitch test
precipitation
benedict’s test (+)
green-orange-red
*Reducing substances
test for lactose and glucose
Rubner’s test
positive rubner’s test
Lactose: brick red with red ppt. (+)
glucose: red color with yellow precipitate
Borchardt’s also known as
Seliwanoff’s
Borchardt’s/Seliwanoff’s tests for
sugars/ fructose
(+) Borchardt’s/Seliwanoff’s test
Red color
lead acetate + ammonia H2O
Rubner’s
benedict’s rgt. composition
CuSO4 +NaCO3 + Na citrate buffer
copper sulfate
sodium carbonate
sodium citrate buffer
tests for Pentose
Bial orcinol
Tauber’s
Fehling’s (+)
Yellow precipitate: (+) glucose
Ictotest
for Bile pigments
- blue to purple mat (+)
- uses Ictotablets
Schales and Schales also known as
Mercurimetric titration
*test for Chloride
Rothera’s test reagents
sodium nitroprusside + ammonium sulfate
Rothera’s test (+)
acetone and AAA = (+) Rose/purple ring
KETONES
Acetoacetate
Acetone
Beta-Hydroxybutyric acid
a test for ketones that uses Na nitroprusside
Legal
Legal test (+)
Acetone = (+) purple/violet AAA = (+) Red
a test for ketones that uses 10% FeCl3
Gerhardt’s
Gerhardt’s (+)
AAA = (+) Bordeaux red color
Qualitative test for protein that uses Exton’s reagent (Na2SO4 + SSA + Dist. H2O)
Sulfosalicylic acid
Biuret test is for
Qualitative test for protein
Quantitative test for Protein
Esbach’s
Kwilecki’s
Tsuchiya’s
Kingsburry-Clark
