Tests Flashcards

0
Q

Purdy’s modification of Heat and HAc test

A

Sat. Soln. NaCl.
Glacial Acetic acid

Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)

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1
Q

Heat and HAc test

A

5% or 10% HAc

Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)

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2
Q

Picric acid test

A

sat. Picric acid

Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)

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3
Q

Osgood haskin’s test

A

50% HAc
Sat. Soln. NaCl.

Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)

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4
Q

Potassium Ferrocyanide Test

A

Glacial HAc
5% to 10% potassium
Ferrocyanide

Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)

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5
Q

Exton’s test ( or sulfosalicylic acid test)

A

Sulfosalicylic acid
Sodium sulfate

Turbidity or white rint

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6
Q

Spiegler’s test

A
30% HAc
Spiegler's reagent: (Sms)
- Succinic acid
- mercuric chloride
- sodium chloride

Turbidity or white ring

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7
Q

Heller’s test

A

Conc HNO3

White ring

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8
Q

Robert’s test

A

Robert’s reagent:

  • magnesium sulfate 7H2o
  • HNO3
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9
Q

Biuret reaction

A

10% NaOH or KOH
10% Cupric sulfate

Violet or rose color

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10
Q

Paper electrophoresis/ cellulose acetate electrophoresis

A

Refer to cc notes

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11
Q

Kwilecki’s modification if esbach’s test

A

10% FeCl
Esbach reagent:
- picric acid
- citric acid

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12
Q

Tsuchiya’s test

A

Tsuchiya’s reagent:

  • phosphotungstic acid
  • 95% alcohol
  • conc. HCL
  • barium sulfate or powdered pumicr
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13
Q

Purdy’s

A

50% HAc

10% potassium ferricyanide

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14
Q

Shanky and Stafford method

A

Tsuchiya’s reagent

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15
Q

Kingsbury and Clark Method

A

3% sulfosalicylic acid

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16
Q

Harrison’s test

A

HAc

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17
Q

Osgood-Haskin’s test

A

HAc, NaCl

18
Q

Bradshaw test

A

Conc. HCl

19
Q

Heal precipitation

A

HAc NaCl

20
Q

Pulnam’s Method

A

2M acetate buffer

21
Q

Toluene sulfonic acid test

A

Toluene sulfonic acid

22
Q

Sulfosalicylic acid test

A

Sulfosal acid

23
Q

Jacobson and miner’s test

A

HAc

24
Q

Protein paper extraction test

A

NaCl
Acetate buffer
Sulfosate acid

25
Q

Method by hepler

A

Glacial Hac

26
Q

Mucicarmine cytologic test

A

Mucicarmine stain

27
Q

Benedict’s test

A

Benedict’s reagent:

  • copper sulfate
  • sodium citrate
  • sodium carbonate

Green to yellow to orange red ppt

28
Q

Fehling’s test

A

Fehling’s rgt:
a = cupric sulfate
B = rochelle salt, KOH

29
Q

Haine’s test

A

Haine’s rgt

30
Q

Trommer’s test

A

10% KOH/NaOH

10% Copper sulfate

31
Q

Nylander’s test

A

Nylander’s rgt:

  • rochelle salt
  • 10% NaOH
  • bismuth subnitrate
32
Q

Moore-heller’s test

A

10% KOH

33
Q

Yeast fermentation

A

Yeast

34
Q

Phenylhydrazine test

A

Phenylhydrazine
Hydrocholride sodium
Acetate

35
Q

Kowarsky’s method

A

Phenylhydrazine
Hydrochloride sodium
Sat. NaCl

36
Q

Benedict’s quantitative test

A

Benedict’s quantitative reagent:

  • copper sulfate
  • sodium carbonate
  • sodium citrate
  • potassium sulfocyanate
  • potassium ferrocyanate
37
Q

Fehling’s quantitative test

A

Fehling’s quantitative reagent

38
Q

Robert’s fermentation test

A

Yeast

Difference in Specific gravity

39
Q

Einhorn’s fermentation

A

Yeast

Based on degree of fermentation

40
Q

Millard-smith micromodification of benedict’s

A

Benedict’s reagent

41
Q

Reagent strip test
Glucose oxidase double
Sequential enzyme reaction

A

Glucose oxidase
Peroxidase
Chromogen buffer

42
Q

Oxygen rate method

Other tests for blood glucose

A

Cc notes