Tests Flashcards
Purdy’s modification of Heat and HAc test
Sat. Soln. NaCl.
Glacial Acetic acid
Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)
Heat and HAc test
5% or 10% HAc
Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)
Picric acid test
sat. Picric acid
Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)
Osgood haskin’s test
50% HAc
Sat. Soln. NaCl.
Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)
Potassium Ferrocyanide Test
Glacial HAc
5% to 10% potassium
Ferrocyanide
Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)
Exton’s test ( or sulfosalicylic acid test)
Sulfosalicylic acid
Sodium sulfate
Turbidity or white rint
Spiegler’s test
30% HAc Spiegler's reagent: (Sms) - Succinic acid - mercuric chloride - sodium chloride
Turbidity or white ring
Heller’s test
Conc HNO3
White ring
Robert’s test
Robert’s reagent:
- magnesium sulfate 7H2o
- HNO3
Biuret reaction
10% NaOH or KOH
10% Cupric sulfate
Violet or rose color
Paper electrophoresis/ cellulose acetate electrophoresis
Refer to cc notes
Kwilecki’s modification if esbach’s test
10% FeCl
Esbach reagent:
- picric acid
- citric acid
Tsuchiya’s test
Tsuchiya’s reagent:
- phosphotungstic acid
- 95% alcohol
- conc. HCL
- barium sulfate or powdered pumicr
Purdy’s
50% HAc
10% potassium ferricyanide
Shanky and Stafford method
Tsuchiya’s reagent
Kingsbury and Clark Method
3% sulfosalicylic acid
Harrison’s test
HAc
Osgood-Haskin’s test
HAc, NaCl
Bradshaw test
Conc. HCl
Heal precipitation
HAc NaCl
Pulnam’s Method
2M acetate buffer
Toluene sulfonic acid test
Toluene sulfonic acid
Sulfosalicylic acid test
Sulfosal acid
Jacobson and miner’s test
HAc
Protein paper extraction test
NaCl
Acetate buffer
Sulfosate acid
Method by hepler
Glacial Hac
Mucicarmine cytologic test
Mucicarmine stain
Benedict’s test
Benedict’s reagent:
- copper sulfate
- sodium citrate
- sodium carbonate
Green to yellow to orange red ppt
Fehling’s test
Fehling’s rgt:
a = cupric sulfate
B = rochelle salt, KOH
Haine’s test
Haine’s rgt
Trommer’s test
10% KOH/NaOH
10% Copper sulfate
Nylander’s test
Nylander’s rgt:
- rochelle salt
- 10% NaOH
- bismuth subnitrate
Moore-heller’s test
10% KOH
Yeast fermentation
Yeast
Phenylhydrazine test
Phenylhydrazine
Hydrocholride sodium
Acetate
Kowarsky’s method
Phenylhydrazine
Hydrochloride sodium
Sat. NaCl
Benedict’s quantitative test
Benedict’s quantitative reagent:
- copper sulfate
- sodium carbonate
- sodium citrate
- potassium sulfocyanate
- potassium ferrocyanate
Fehling’s quantitative test
Fehling’s quantitative reagent
Robert’s fermentation test
Yeast
Difference in Specific gravity
Einhorn’s fermentation
Yeast
Based on degree of fermentation
Millard-smith micromodification of benedict’s
Benedict’s reagent
Reagent strip test
Glucose oxidase double
Sequential enzyme reaction
Glucose oxidase
Peroxidase
Chromogen buffer
Oxygen rate method
Other tests for blood glucose
Cc notes
