Test3

Question 1

Lin14 encodes

Answer 1

nuclear protein

Question 2

Lin14 gain of function is

Answer 2

3’ UTR deleted so repressor can’t bind

always ON in L1 and L2

Question 3

Lin 14 loss of function

Answer 3

OFF in L1 and L2

Question 4

Lin4 is A

Answer 4

noncodin microRNA

Question 5

Lin4 encodes for

Answer 5

trans acting repressor that binds to Lin14 at 3’UTR to make a bulged complex

Question 6

Lin4 loss of function is a lin 14

Answer 6

gain of function

Question 7

The two types of RNAi (small RNA) are

Answer 7

micro RNA and small interfereing (si)RNA

Question 8

The source of siRNA is
The source of miRNA is

Answer 8

siRNA: transgenic source of dsRNA
miRNA: genic source of dsRNA

Question 9

siRNA complementarity is
mi complementarity is

Answer 9

siRNA: specific target with perfect comp. to original genes from which they were transcribed

miRNA: imperfect comp = multiple targets

Question 10

siRNA affects target by:
miRNA affect target by:

Answer 10

siRNA: target degraded
miRNA: target RNA is still present but are inhibited from translation

Question 11

use of siRNA:
use of miRNA:

Answer 11

siRNA: aritificial tool for knowckdown of gene expression in expmt
miRNA: eukaryotes use as endogenous mech. of gene regulation

Question 12

siRNA and miRNA are similar by?

Answer 12

1. triggered by dsRNA
2. diced by Dicer into small fragments
3. small fragments pair with AGO to make RNA-induced silencing complex
4. targets RNA by complmentarity of RNAi and target RNA
5. in eukaryotes
6. evolved as innate immunte system to suppress viruses and transposons

DICEDAR

Question 13

What is the role of small RNA in RNAi?

Answer 13

targeting AGO + RISC complex to the target RNA for silencing translation

Question 14

how can we use RNAi in research?

Answer 14

transform specific dsRNA into eukaryotic cell for specific post-transcriptional silencing of target gene (endogenous RNAi machery)