Test one

Question 1

whats the capacity for p-10

Answer 1

.5 microliters to 10 microliters

Question 2

whats the accuracy for p-10

Answer 2

.2 microliters

Question 3

whats the capacity for p-20

Answer 3

2 to 20 microliters

Question 4

whats the accuracy for p-20

Answer 4

.2 microliters

Question 5

whats the capacity for p-200

Answer 5

20 microliters to 200 microliters

Question 6

whats the accuracy for p-200

Answer 6

2 microliters

Question 7

whats the capacity for p-1000

Answer 7

200 microliters to 1000 microliters

Question 8

whats the accuracy for p-1000

Answer 8

20 microliters

Question 9

how to find percent error

Answer 9

(measured-avg)x 100

Question 10

what are the units for molarity

Answer 10

mol/L

Question 11

what are the units for molecular weight

Answer 11

g/mol

Question 12

molecular weight can also be expressed in

Answer 12

daltons

Question 13

when two components at the same concentration are mixed whats the dilution

Answer 13

no dilution

Question 14

what is spectrophotometry

Answer 14

measurement of how much a chemical substance absorbs or transmits

Question 15

what is a wavelength

Answer 15

distance between two consecutive crests or troughs

Question 16

what is amplitude

Answer 16

height of wave crest above the reference line, shows intensity of wave

Question 17

what is frequency

Answer 17

number of oscillations per second

Question 18

shorter wavelength has what type of energy

Answer 18

high

Question 19

longer wavelength has what type of energy

Answer 19

low

Question 20

visible light wavelength range

Answer 20

340 to 800

Question 21

UV wavelength range

Answer 21

200 to 340

Question 22

when the electron falls back into the ground state, the excess energy is lost as

Answer 22

heat or fluorescence

Question 23

white light is composed of

Answer 23

all colors

Question 24

if an object absorbs all wavelengths of visible light, it appears…

Answer 24

black

Question 25

if the absorption spectra is showing for a certain color, what happens

Answer 25

the complimentary color is shown

Question 26

absorbance is proportional to what

Answer 26

length of sample holder and concentration

Question 27

units for extinction coefficient

Answer 27

M-1, cm-1

Question 28

purpose of bradford assay

Answer 28

measure protein concentration

Question 29

units of dalton are

Answer 29

g/mol

Question 30

you can estimate molar extinction coefficient usin

Answer 30

amino acid composition

Question 31

how is cystine formed

Answer 31

when two cysteine residues form a disulfide bond

Question 32

if cystine is in cytosol what happens

Answer 32

does not occur

Question 33

what are the four chromogenic assay methods for determining protein concentration

Answer 33

biuret method, lowry assay, BCA method, Bradford assay

Question 34

what are the two wavelengths that proteins absorb

Answer 34

220, 280

Question 35

absorbance for a protein depends on what three things

Answer 35

tryptophan, tyrosine and cystine

Question 36

Biuret method

Answer 36

uses copper, no worries about free aa, no aromatic aa, not very sensitive

Question 37

lowry assay

Answer 37

2 step, uses copper, very sensitive, many compounds can interfere

Question 38

BCA method

Answer 38

uses copper, one step, many compounds can interefere

Question 39

bradford assay

Answer 39

uses blue dye, sensitive, not dependent on aromatic aa

Question 40

wavelength of nucleic acid absorbtion

Answer 40

260 nm

Question 41

what interrupts resonance and limits absorbance in DNA

Answer 41

h bonds

Question 42

denaturing of RNA/DNA causes increased what

Answer 42

absorbance