Test One Flashcards
Serum is the liquid portion of the blood without :
Fibrinogen
A centrifuge must always be balanced which means the tubes must be
Placed opposite each other or the centrifuge must be full when it is turned on
Serum sits to clot hence no :
Fibrinogen
Centrifuge for 10 to
15 mins
Anticoagulant
A chemical that inhibits clotting
Buffy coat
Thin layer WBC’s & platelets between separated cells in plasma
Centrifuge
Instrument that spins at high rpm to separate substances
Hemolysis
Breakdown of blood
Fibrinogen
Protein present in blood that is essential for clotting
Plasma
Liquid portion of whole blood (unclotted blood) that contains all the clotting factors.
Clear yellowish
Hematoma
Swelling or mass of clotted blood under the skin
Bifurcation
Separated into two branches
Serum
Liquid portion of blood that does not contain any clotting factors
Clear yellowish
Syringe advantages
Comes in various sizes, able to control amount of blood, best for small spidery veins.
Syringe disadvantages
Slower, smaller quantities.
Vacutainer advantages
Faster, large volume of blood, multiple sampling (ability to fill multiple tubes in one stick)
Vacutainer disadvantages
Less control, veins collapse under vacupressure
Where is the tourniquet applied
Two to three inches above antecubicle site
How long is tourniquet applied
Never leave on longer than one minute
What is the preferred site for a blood draw
Antecubicle veins, median cubicle, cephalic & basilic
Why r antecubicle veins preferred site for blood draw
Large, close to surface, easily accessible
Supplies required to perform venipuncture
Tidy towel, cotton or gauze, bandage, 20-21 gauge 1 inch needle, tube holder & alcohol
Serum is clear yellowish and contains no
Fibrinogen
Serum tubes
SST or red
Plasma is clear yellowish and contains all
Clotting factors intact
Plasma tubes
Lavender, EDTA (anticoagulant)
Lavender tubes test plasma
Tests done are:
CBC, hematocrit, hemoglobin
Red SST tubes test serum.
Clotting additive or nothing.
Tests:
Chemistries, electrolytes, and cholesterol
Grey tubes sodium fluoride used for
Used for fasting or random glucose (gtt)
Never draw blood above an IV, always below, ask nurse to :
Turn off IV for 2 mins b4 drawing blood, make sure nurse turns IV back on after the draw