Test For Protein Flashcards
What is the first step in conducting the Biuret test for protein?
Introduce 3cm3 food solution into a test tube using a clean syringe.
Ensure that the syringe is clean to avoid contamination.
What volume of Biuret solution should be added to the test tube?
3cm of Biuret solution using a clean plastic pipette.
The use of a clean pipette is essential to prevent interference in the test results.
What does a blue color indicate in the Biuret test?
Negative result (Proteins are absent)
A blue color means no proteins are detected in the sample.
What color indicates a positive result for proteins in the Biuret test?
Deep purple
A deep purple color shows that proteins are present in the solution.
What should be done after adding Biuret solution to the food solution?
Gently shake the test tube and observe.
Gentle shaking helps to mix the solutions properly for an accurate test.
What change occurs in the color of the solution when proteins are present?
The pale blue color turns to violet.
This color change is a key indicator of protein presence.
What does a negative result for protein look like in the Biuret test?
The solution stays pale blue.
A consistent pale blue color indicates the absence of proteins.
