test for non reducing sugars Flashcards

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1
Q

how do you carry out the test for non reducing sugars?

A
  • carry out benedict’s test on a small sample to confirm a negative result
  • hydrolyse another sample of the sucrose by boiling it with dilute acid eg HCL
  • when cooled, add dilute sodium hydroxide solution (NaOH) to neutralise the acid
  • add benedict’s solution and heat in a water bath for 5 minutes
  • a positive brick red colour indicates a non reducing sugar (sucrose) was originally present
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1
Q

what is an example of a non reducing sugar?

A

sucrose

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