test for non reducing sugars

Question 1

how do you carry out the test for non reducing sugars?

Answer 1

• carry out benedict’s test on a small sample to confirm a negative result
• hydrolyse another sample of the sucrose by boiling it with dilute acid eg HCL
• when cooled, add dilute sodium hydroxide solution (NaOH) to neutralise the acid
• add benedict’s solution and heat in a water bath for 5 minutes
• a positive brick red colour indicates a non reducing sugar (sucrose) was originally present

Question 1

what is an example of a non reducing sugar?

Answer 1

sucrose