test for non reducing sugars Flashcards
1
Q
how do you carry out the test for non reducing sugars?
A
- carry out benedict’s test on a small sample to confirm a negative result
- hydrolyse another sample of the sucrose by boiling it with dilute acid eg HCL
- when cooled, add dilute sodium hydroxide solution (NaOH) to neutralise the acid
- add benedict’s solution and heat in a water bath for 5 minutes
- a positive brick red colour indicates a non reducing sugar (sucrose) was originally present
1
Q
what is an example of a non reducing sugar?
A
sucrose