test for leaf areas that contain/contained starch

Question 1

1.

Answer 1

put the leaf into boiling water to denature the enzymes present in the leaf

Question 2

2.

Answer 2

put the boiled leaf in a boiling tube containing 3cm3 of ethanol to decolourise the leaf. Place the boiling tube in a water bath of 50.c for 10 minutes
MAKE SURE TO NOT DIRECTLY HEAT THE BOILING TUBE CONTAINING THE ETHANOL BC ETHANOL IS VERY FLAMMABLE

Question 3

3.

Answer 3

after 10 min, remove the leaf and place it back into the water bath and swish it around to soften the leaf and make it more permeable to iodine

Question 4

4.

Answer 4

after about 1 min take it out and spread it evenly into a whit tile. add 2-3 drops of iodine

Question 5

results?

Answer 5

the areas that are blue black contains starch

the areas that remain brown containED starch as the starch already has been converted to glucose

Question 6

this experiment plan will only work for plants that were exposed to …?

Answer 6

sunlight for a few hours