Test 2 Revision - Stains

Question 1

PAS

Answer 1

Pink - glycogen, neutral and weakly acid mucins, collagen, basement membrane
Nuclei - blue

Question 2

PAS/D

Answer 2

Same as PAS however, glycogen has been digested by the diastase and is therefore absent
Often used as a control for PAS

Question 3

PAS/Alcian Blue

Answer 3

Blue - acid mucins
Pink - neutral mucins
Nuclei - pale blue

Question 4

Alcian Blue/VG

Answer 4

Turquoise - acid mucins
Red - collagen
Muscle and RBCs - yellow

Question 5

Toluidine Blue

Answer 5

Dark blue - H.pylori organisms

Pale blue - background

Question 6

Picro-Mallory

Answer 6

Blue - collagen
Red - fibrin
Yellow - RBCs
Blue/black - nuclei

Question 7

Periodic Acid Methenamine Silver

Answer 7

Used for basement membranes
Black - basement membranes, reticular fibres
Lime green - nucleus and background

Question 8

Oil Red O

Answer 8

Red - triglycerides

Nuclei - blue

Question 9

CK7

Answer 9

Brown cytoplasmic staining of normal and neoplastic tissue above diaphragm

Question 10

CK20

Answer 10

Brown cytoplasmic staining of epithelium and tissue below diaphragm

Question 11

EMA

Answer 11

Brown cytoplasmic and membrane staining of normal and neoplastic epithelium

Question 12

TTF-1

Answer 12

Brown nuclear staining

Selectively expressed in lung and thyroid