TEST 2. I

Question 1

What do you call a complex prosthetic group or cofactor with functional

Answer 1

Holoenzyme

Question 2

What do you call a reaction whose rate is dependent of the enzyme

Answer 2

Zero order kinetics

Question 2

Which of the following enzymes is devoid both in liver and RBC

Answer 2

CK

Question 3

what kind of enzyme specificity is it when only one of the isomers actes as a substrate for an enzyme action

Answer 3

stereochemical specificity

Question 3

The following but one is true with regards to the criteria for selecting enzymes, measure in serum for diagnostic purposes

Answer 3

None of the above

Question 4

Which of the following enzymes catalyzes the splitting of D-fructose 1, 6 diphosphate to DHAP and GLAP

Answer 4

Aldolase

Question 5

Which of the following is true about clinically common alteration in liver enzyme activities

Answer 5

all of the above

Question 6

which of the following enzymes would be used to determine prostate cancer

Answer 6

LD

Question 6

The following but one represents activators of ALP

Answer 6

Borate

Question 7

Elevation in 5 nucleotidase or y glutamyltransferase together with ALP suggest that the elevated ALP is of liver not borne

Answer 7

True

Question 8

AST

Answer 8

Aspartate aminotransferase

Question 9

ALT

Answer 9

Alanine aminotransferase

Question 10

GGT

Answer 10

gamma glutamyltransferase

Question 10

ALP

Answer 10

Alkaline Phosphatase

Question 11

ALD

Answer 11

Aldolase

Question 12

LD

Answer 12

lactate dehydrogenase

Question 13

CK

Answer 13

Creatine kinase

Question 14

NAD

Answer 14

Nicotinamide adenine dinucleotide

Question 15

EDTA

Answer 15

Ethylenediamine tetra acetic acid

Question 16

explain why children have twise ALD activit6as that of aldult

Answer 16

Children need energy for cell growth and development, so aldolase provide energy to the cells

Question 17

Which free factors would you consider any selection of enzyme for measurement in Serum for diagnostic purposes

Answer 17

1. Distribution of enzymes in a various tissues
2. Knowledge of the location of Enzymes in intracellular spaces
3. clearance way and the late at which the enzyme activity disappears from the blood

Question 17

Briefly describe the principle behind Hexokinase (HK) glucose 6 phosphate heterogeneous G6PD couples assay for CK analysis

Answer 17

1.Firstlyl creatine phosphate reacts with ATP to yield creatine and ATP in the presence of creatine kinase
2. Then ATP is reacting with glucose to produce glucose six phosphate and ATP in the presence of hexokinase
3. Glucose 6 Phosphate is reacted with NADP+ s to produce 6 phosphogluconate + NADPH + H+ in the presence of glucose 6 phosphate the dehydrogenase

Question 17

explain 2 ways why enzymes are tools of diagnostic importance

Answer 17

1. because they help in detecting injury of tissues that makes up the enzymes
2. Because they help in identifying abnormalities or absence of enzymes that may cause disease