Test 2 Flashcards
whole blood
common specimen type–collected via anti-coagulant—includes RBCs, WBCs, & platelets
serum
common specimen type–remaining fluid after blood has clotted & clots & platelets are removed by spinning
plasma
common specimen type–straw-colored blood component containing H2O, inorganic electrolytes, & clotting factors
blood cells
common specimen type–separated from anti-coagulated whole blood for analysis
erythrocytes
common specimen type–RBCs
leukocytes
common specimen type–WBCs
blood spot
common specimen type–dried whole blood from finger or heel prick—put on paper & tested for hormones & others (ex: infant PKU screening)
other tissues
common specimen type–from scrapings or biopsy samples
urine
common specimen type–concentrate of excreted metabolites
feces
common specimen type–detects presence of nut. not absorbed or used to determine gut flora composition
breath test
less common specimen type–evaluates met., use, & malabsorption of sugars
hair & nails
less common specimen type–identifies exposure to certain metals—if prob is evident here, it is severe
saliva
less common specimen type–evaluates functional adrenal stress & hormone levels
sweat
less common specimen type–detects sweat chloride levels to determine presence of cystic fibrosis
static assay
measures actual nut. level in specimen—gives most recent reading—limitation: influenced by decent dietary intake—ex: check Fe, folic acid, & Vit. B12 for anemia
functional assay
gives quantitative measure of biochem. or physiologic activity that depends on specific nut.—tests function —ex: serum ferritin (transportation prob?)
basic met. panel (BMP)
clinical chem. panel—8 tests for screening
comprehensive met. panel (CMP)
clinical chem. panel—8 BMP tests + 6 more tests—is most commonly ordered
complete blood count (CBC)
count of cells in blood & description of RBCs—tells what is in cells
urinalysis
screening test or diagnostic tool—detects substances (glucose, protein, albumin, & ketones) or cellular material in urine associated w/ dif. met. & kidney disorders
albumin
hepatic transport protein—transports major blood constituents, hormones, enzymes, meds, min., ions, FAs, AAs, & metabolites—maintains colloidal osmotic pressure—↓ means edema—1/2 life is 18-21 days (does NOT reflect current protein intake)
pre-albumin (PAB)
hepatic transport protein—transports thyroid hormones—1/2 life is 2 days→used as indicator of protein status–↓ is related to: inflammation, protein-wasting disease of GI tract, & Zn def.
retinol-binding protein (RBP)
hepatic transport protein—shortest 1/2 life (12 hrs)—binds & transports retinol (Vit. A)
transferrin
hepatic transport protein—transports Fe to bone marrow to produce Hgb—1/2 life is 8 days
(nut.) anemia
↓ in # RBCs / unit of blood volume OR ↓ in [Hbg] of blood to below needed level
microcytic anemia
“small cells”—Fe def.
macrocytic anemia
folate or Vit. B12 def—”large cells”
normocytic anemia
chronic & inflammatory diseases—*does NOT respond to Fe supp.
lab tests for Fe-def. anemia:
- Hct (packed cell volume)
- Hbg, serum ferritin
- effects of inflammation
- serum Fe
- total Fe-binding capacity (TIBC)
(liver of any animal is great source of Fe)
megaloblastic anemia
folate def.
pernicious anemia
Vit. B12 def. (found in meat products only→vegans must inject)—activated by intrinsic factor (IF) in stomach—is absorbed in ↓er colon
hematocrit (Hct)
measure of % RBCs in total blood volume—is usually 3x ↑er than [Hgb]—affected by extremely ↑ WBC count & hydration status
hemoglobin (Hgb)
measure of total amt. Hgb in peripheral blood
ferritin
Storage protein that sequesters Fe normally gathered in liver, spleen, & marrow—synthesis ↑ w/ inflammation
serum Fe
amt. circulating Fe bound to transferrin—poor index of Fe status
total Fe-binding capacity (TIBC)
measure of all proteins available to bind mobile Fe–depends on # free binding sites on plasma transferrin (Fe-transport protein)
Schilling Test
detects defects in Vit. B12 absorption (is gold standard for measuring Vit. B12)
serum retinol
measures Vit. A status
plasma-25-hydroxyvitamin D (25-OH-D3)
measures Vit. D status–Vit. D def. can lead to 2° malabsorption of Ca
Hgb A1C
does NOT reflect more recent ∆glucose levels–does NOT diagnose DM but reflects control of glucose
lipi indices of CV risk:
- ↑ LDL
- ↑ triglycerides
- ↑ apoprotein B
- ↑ Hs-CRP
- ↑ serum homocysteine
oxidation
break-down of cells b/c stress (is fought by anti-oxidants: Vit. A, Vit. C, Vit. E, & Se)–markers are ↓ed by carotenoids & smoking cessation
IBW (male)
IBW (male) = 106 lbs. for 5’ + 6 lb./in. ± 10%
IBW (female)
IBW (female) = 100 lbs. for 5’ + 5 lb./in. ± 10%