test

Question 1

Peripheral mononuclear cells comprise

Answer 1

Monocytes + lymphocytes (both T and B cells) and a small percentage of other immune cells such as dendritic cells

Question 2

proper expansion of T lymphocytes in vitro requires

Answer 2

Cytokines such as IL-2
Co-stimulatory signal
Activation signal

Question 3

Dendritic cells can be generated in vitro from

Answer 3

Monocytes

Question 4

Activation of T cells can be achieved by using

Answer 4

Magnetic beads coated with antibodies
K562 cell line serving as artificial antigen presenting cells (aAPC)
Autologous dendritic cells

Question 5

According to the traditional concept, macrophages are classified into;

Answer 5

Proinflammatory (m1), non activated (m0) or anti-inflammatory (m2)

Question 6

Flow cytometric analysis is commonly used fro identification and description of leukocytes for

Answer 6

For research
For clinical diagnosis (i.e inflammation)
For disease monitoring (i.e leukaemia, lymphoma)

Question 7

Types of sample suitable for flow cytometry are

Answer 7

Mononuclear cells isolated from the blood
Bone marrow aspirate
Cell cultured in vitro ( Have to be processed to from cell suspension)

Question 8

BAsed on forward scatter and side scatter of flow cytometer we ca analyze

Answer 8

Cell size
Cell granularity
Cell number

Question 9

The parameters analyzed by flow cytometry include

Answer 9

Physical properties of cells; size, granularity, DNA Content
Cell type
Cell lineage (B cells, T cells, NK cells)

Question 10

using specific antibody staining of lymphocytes for flow cytometry we can assess the expression of;

Answer 10

Surface markrers such as CD molecules
Cytokines
Transcription factors

Question 11

CD3 is a marker of

Answer 11

T cells

Question 12

Flow cytometry is well suited for which analysis

Answer 12

Cell DNA content analysis
Cell proliferation analysis
Cell death analysis

Question 13

Chimeric antigen receptor (CAR)

Answer 13

Is genetically modified T cell Receptor (TCR) that do not occur in nature
Can recognise specific tumor-associated antigens
Is chimeric because it combines both antigen binding and T cell activating function into a single receptor

Question 14

What is anticancer immunotherapy?

Answer 14

The method to treat cancer by harnessing immune cells to fight against cancer cells
The way of improving the immune systems natural ability to eradicate cancer cells
The type of cancer treatment that may involve cytokine therapy, cellular therapy, antibody therapy (especially immune checkpoint blockade), and therapeutic vacccines

Question 15

SCIENTIFIC ARTICLE in the field of life sciences and medicine should contain

Answer 15

Research hypothesis
Description of materials and method used
Quantitative Results and Discussion