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Histo > Test 1 Fixateur > Flashcards

Test 1 Fixateur Flashcards

1
Q

Ingrédients formaldéhyde 10%

A

1 partie formol 100% (37-40%) et 9 partie d’eau

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2
Q

Ingrédients formol zinc

A

Sulfate de zinc / formaldéhyde 40%

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3
Q

Quel sont le aldehyde

A

Formaldéhyde / paraformaldéhyde / glutaraldehyde

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4
Q

Coag ou non -alcool

A

Goag

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5
Q

Coag ou non-sels de zinc

A

Coag

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6
Q

Coag ou non-chlorure mercurique

A

Coag

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7
Q

Coag ou non - trioxide de chrome

A

Coag

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8
Q

Coag ou non - acide picrique

A

Coag

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9
Q

Coag ou non - formaldehyde

A

Non coag

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10
Q

Coag ou non - glutaraldehyde

A

Non coag

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11
Q

Coag ou non - tetroxide d ‘osmium

A

Non coag

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12
Q

Coag ou non bichromate de potassium

A

Non coag

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13
Q

Coag ou non - acide acetique

A

Non coag

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14
Q

Coagulant=?

A

Formation d’un reseau de fibres

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15
Q

Non coagulant=?

A

Formation de gel

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16
Q

Additifs ou non-formaldehyde

A

Additifs

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17
Q

Additifs ou non- glutaraldehyde

A

Additifs

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18
Q

Additifs ou non- acide picrique

A

Additifs

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19
Q

Additifs ou non- chlorure mercurique

A

Additifs

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20
Q

Additif ou non- trioxide de chrome

A

Additifs

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21
Q

Additifs ou non- tetroxyde d’osmiume

A

Additifs

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22
Q

Additifs ou non- sulfate/chlorure de zinc

A

Additifs

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23
Q

Additifs ou non- ethanol

A

Non additifs

24
Q

Additifs ou non- methanol

A

Non additifs

25
Q

Additifs ou non- acide acetique

A

Non additifs

26
Q

Additifs ou non- acetone

A

Non additifs

27
Q

Tolerant ou non- formol

A

Tolerant

28
Q

Tolerant ou non bouin

A

Intolerant

29
Q

Deuxieme fixation utilite:

A

Preserver certains substances tissulaires particulieres
Augmenter l’affinite du tissu pour le colorant

30
Q

Postmordancage:

A

Apporte au tissu des groupements chimiques qui ont une affiniter pour le colorant et pour le tissu

31
Q

A quel temperature pour fixer?

A

Temperature piece

32
Q

Pourquoi couper/ouvrire le grosse piece? (Poumon, intestin,uteruse)

A

Pour que le fixateur rentre mieu das la piece

33
Q

Combien de fixateur?

A

20x plus que le volume de la piece

34
Q

Avantage fixateur plus long a traverser

A

Plus vite a fixer

35
Q

Desavantage fixateur plus vite a traverser

A

Plus long a fixer

36
Q

Quel ph pour fixer

A

Ph=6-8

37
Q

Pression oncotique:

A

Assurer un equilibre hypertonique vs hypotonique

38
Q

Quel fixatif ne reagit pas avec ADN/ARN

A

Formaldehyde

39
Q

Meilleur fixatif pour elements nucleaires

A

Alcool-acetique et carnoy

40
Q

Fixateur qui augmente basophilie

A

Acide acetique

41
Q

Structure tissulaire negative acide ou basique

A

Acide

42
Q

Structure tissulaire positive acide ou basique

A

Basique

43
Q

Quel fixateur pour ne pas perdre les lipides

A

Tetroxyde d’osmium et acide chromique

44
Q

Quesque le xylene fait au lipide

A

Les dissous

45
Q

Meilleur fixateur pour lipide

A

Congelation

46
Q

Combien de temps la formaldehyde prend pour fixer

A

24-48h

47
Q

Avantage glutaraldehyde

A

Rapide pour petite piece (biopsi)

48
Q

Peut on utiliser acide acetique seul

A

Non (jamais seul)

49
Q

Quel fixateur est bannie au canada et quel est son avantage

A

Chlorure mercurique/ rehausse coloration

50
Q

Le B-5 est remplacer par quoi

A

B plus

51
Q

Ingrediant B plus

A

Formol zinc+acide acetique

52
Q

Ingredients bouin

A

Acide picrique+acide acetique glacial+formaldehyde 40%

53
Q

Ingredients gendre

A

Alcool 95% sature a l’acide picrique+acide acetique glacial+formaldehyde 40%

54
Q

Ingredients hollande

A

Acetate de cuivre+acide acetique glacial+formaldehyde40%+acide picrique

55
Q

Ingredients zenker

A

Clorure mercurique+bichromate de K+eau+acide acetique

56
Q

Ingredients helly

A

Chlorure mercurique+bichromate de K+Eau+formaldehyde 40%

57
Q

Hollande est meilleur que…

A

Bouin

Histo (6 decks)

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