TEST 1 ASDOH MICRO Flashcards
What are the 4 direct tests to identify microorganisms
Direct microscopic analysis, Microbial culture physiology and morphology, Antigenic analysis (serology), Genetic analysis (PCR)
The indirect way to identifying a microbe is through what process
The final way to identify an infectious microbe is to look for the appearance of antibodies against the microbe in the infected individual. Example would be how they check for HIV
What are the 5 ways to identify a microorganism
Direct microscopic analysis
Microbial culture, physiology and morphology
Antigenic analysis (serology)
Genetic analysis (PCR)
Appearance of antibodies angering the microbe of infected individual
how does microbial culture, physiology and morphology test
Narrows down the possibilities of bacteria by specific oger plates that will only allow the growth of a certain type of bacteria,
Antigenic analysis (serology)
Used in cases of strep throat to find microbial antigens by use of antibodies
Genetic analysis
Most errors, use of PCR checks the amount of DNA and RNA
Microbial agents of disease can be divided into three broad groups
Normal flora- generally helpful not pathogenic
opportunists
pathogens
Opportunists are
normal flora bacteria that under unusual circumstances such as immunosuppression, Impairment of either the innate and or adaptive immune system. etc.
Pathogens
typically cause disease when present and are note generally normal flora
Diagnostic microbiology
requires identification of potential microbial causes of disease either by direct assay of tissue or isolation and culture of infectious agent followed by identification.
Gram positive example
strep throat
Acid fast stain example
lepeardcy TB microplasma
India Ink stains for
capsules of viruses
KOH prep stains for
Fungi, kills everything but fungus
FTIC ecample
ELISA serology Antibiodics
