Test 1 Flashcards
Leeuwenhoek
Used microscope to see microorganisms.
• first saw bacteria
Needham
In favor of spontaneous generation
Spallanzani
Reported results that contradicted needham findings which were in favor of spontaneous generation
Pasteur
Used swan necked flasks that remained free of microbes disproved the theory definitively.
4 things to know him by:
Swan necked flasks, flasks opened, proved the spontaneous generation was false, fermentation
Koch
Etiology, the study of the causation of disease.
Buchner
Demonstrated the presence of enzymes, cell produced proteins that promote chemical reactions such as fermentation.
Biochemistry- study of metabolism
Gram
Developed staining techniques involving application of a series of dyes that leave some microbes purple and some pink.
Gram stain: gram positive and gram negative
Semmelweis
Hand washing
Nightingale
Founder of modern nursing
Lister
Use of chemicals on surgical
Chemicals to kill Bacteria
Chemotherapy- treating chemicals
Snow
Cholera epidimic
Epidemiology- study of occurrence, distribution, and spread of disease in humans
Jenner
First vaccine
Immunology- body’s specific defenses against pathogens
Smallpox, virus
Kochs postulates
- The suspected causative agent must be found in every case of the disease and be absent from healthy host.
- The agent much be isolated and grown outside the host
- When the agent is introduced into a healthy, susceptible host, the host must get the disease
- The same agent must be reisolated from the disease experimental host
Fermentation, who did experiments to prove it?
Chemical breakdown of a substance by bacteria, yeasts, or other microorganisms.
The process of fermentation involved in the making of beer, wine, and liquor, in which sugars are converted to ethyl alcohol
•Pasteur proved it
Enzymes, what do they do?
- protein catalysts in cells
- biological molecules(typically protein) that significantly speed up the rate of virtually all of the chemical reactions
In favor of spontaneous generation
Aristotle
Needham
Against: Redi, spallanzani, Pasteur
Spontaneous generation- living things originate from non living
Spontaneous generation
Living things originate from non living things.
Proposed by Aristotle
Redi
Against spontaneous generation
Pasteur experiments
4 questions
- Is spontaneous generation possible?
- Fermentation?
- Disease causing?
- Preventing infection/disease?
•swan neck flasks, flasks opened, proved the spontaneous generation was false, fermentation: by microbes
Facultative anarobes
Microorganisms which can live with or without oxygen
Biochemistry
The study of metabolism.
Microbial genetics
The study of inheritance in microorganisms.
Molecular biology
Combines aspects of biochemistry, cell biology, and genetics to explain cell function at the molecular level. Genome sequencing
Recombinant DNA technology- genetic engineering
Involved the manipulation of genes in microbes, plants, and animals for practical applications, such as the development of pest resistant crops and the treatment of disease.
Gene therapy
The use of recombinant DNA ( DNA composed of genes from more than one organism) to insert a missing gene or repair a defective gene in human cells.
Immunology
Study of the body’s specific defenses against pathogens
Epidemiology
The study of the occurrence, distribution, and spread of disease in humans
The main questions scientist were trying to answer during the Golden age of microbiology
- Is spontaneous generation is microbial life possible?
- What causes fermentation?
- What causes disease?
- How can we prevent infection and disease?
Eukaryotes
Are organisms whole cells contain a nucleus composed of genetic material surrounded by a distinct membrane
Prokaryotes
Unicellular microbes that lack a true nucleus
Prokaryotes
No nucleus
Unicellular
Asexual
Bacteria: most are non pathogenic
Cell wall: made of peptidoglycan
Archeae: all of them are non pathogenic
Cell wall: not peptidoglycan
Eukaryote
Have a nucleus
Uni/multicellular
Asexual/sexual
Fungi: cell walls: chitin
Two types: yeast:unicellular
Molds: multicellular
Protozoa:unicellular
First animals/ no cell wall
Motile-pseudopodia
•algae: photosynthetic
Diatoms: unicellular/ produce most D2 on earth
•kelps: multicellular/ gelatinous fell walls
Viruses
Acellular
Cellular parasites
Not visible with light microscope
DNA or RNA: genetic material, enclosed by a capsid: protein coat
Pathogen
Disease causing
Three particles or atom and their charges?
- electrons: negatively charged
- protons:positively charged
- neutrons: uncharged/neutral
What is responsible for interactions in chemical reactions?
valence Electron
Ionic bonds
When two atoms with vastly different electronegativities approach each other, the atom with the high electronegativity will strip one or more electrons from the valence shell of the other.
• opposite charges of cations and anions attract each other strongly to form an ionic bond
Polar-covalent
Bonds with an unequal sharing of electrons.
Non polar covalent
Neither nucleus acts as a pole to exert an unequal pull
Covalent bond
The sharing of a pair of electrons by two atoms
- polar-unequal
- nonpolar- equal