term test 2 Flashcards
kidney location
- located at dorsal lumbar area outside of the abdomen , between peritoneum and dorsal abdominal muscles outside the peritoneal cavity
- right kidney more cranial than the left in most domestic animals
gross anatomy of kidney
- bean-shaped structure
- in cattle , it is lobulated
- hilus
- renal pelvis
- renal cortex
- renal medulla
- calyx
hilus - kidney
- indented area in medial side
- where ureters , nerves , blood and lymph vessels enter and leave the kidney
renal pelvis - kidney
- funnel-shaped area
- beginning of ureter
- collects urine produced by renal cortex before it travels through the ureter into the bladder
renal cortex and renal medulla - kidney
renal cortex ;
- outer portion of kidney
renal medulla ;
- inner portion around renal pelvis
calyx / calyces - kidney
- cup-like structure
- formed when renal pelvis extends into the medulla pyramid
- directs and collects fluids into renal pelvis
microscopic anatomy of kidney
- basic functional unit : nephron
each nephron consists ;
- renal corpuscle
- proximal convoluted tubule (PCT)
- loop of henle (LOH)
- distal convoluted tubule (DCT)
renal corpuscle -> PCT -> LOH -> DCT -> urine
- some parts of a nephron eg renal corpuscle are only in the cortex while others eg loop of henle is only in the medulla
renal corpuscle
- located in renal cortex
- glomerulus / glomerular tuft ; cluster of capillaries
- bowman’s capsule ; surrounds the glomerulus to hold the capillaries
- capsular space / bowman’s space ; between capsule and glomerulus , there is a space
- filters blood in the first stage of urine formation
- fluid that is filtered OUT of blood through capillaries and into capsular space = glomerular filtrate
blood supply into glomerulus
- renal artery enters kidneys at the hilus , dividing into smaller arteries and even smaller arterioles
- afferent glomerular arterioles carries blood into glomerular capillaries of renal corpuscle
- glomerular capillaries filters some plasma out of the blood into the bowman’s space and it becomes glomerular filtrate
- efferent glomerular arterioles receives blood from glomerular capillaries
- A goes in , E goes out
proximal convoluted tubule (PCT)
- continuation of capsular space
- twisting path through the cortex
- when glomerular filtrate enters the PCT from the capsular space , it becomes tubular filtrate
- where 65% of reabsorption takes place
loop of henle (LOH)
- long , u-shaped portion of the tubule
- descends from PCT into medulla (descending LOH)
- turns and heads upwards back into the cortex (ascending LOH)
distal convoluted tubule (DCT)
- DCT is the continuation of the ascending LOH
- DCT of all nephrons will drain urine into collecting ducts
- collecting ducts carries tubular filtrate (which is now waste products only / urine) through the medulla and empties into renal pelvis
- primary site of action of ADH (antidiuretic hormone)
reabsorption in the tubules is possible because …
- efferent glomerular arterioles divide to form peritubular capillaries (small capillaries that surround the tubules)
- allows oxygen supply to the tubules , tubular reabsorption , tubular secretion
- peritubular capillaries will converge at the end to form a larger vein (renal vein)
- renal vein leaves the kidney at the hilus to join the caudal vena cava
3 steps of urine formation
- kidney filters unwanted substances to excrete them
1. glomerular filtration
2. tubular reabsorption
3. tubular secretion
glomerular filtration
- glomerular capillaries are permeable (not enough to allow blood cells and large proteins to pass through)
- plasma proteins and substances such as calcium that are bound to them are unable to pass
- fluids leave bloodstream into capsular space
- in glomerular capillaries , blood pressure is very high and forces some plasma out into capsular space
- glomerular filtrate = primitive urine (first stage)
glomerular filtrate
- fluid that passes through glomerular capillaries into capsule space
- any product that is smaller than plasma protein or freely dissolved in plasma appears in glomerular filtrate
- eg sugars , amino acids , toxins
- glomerular filtrate in the capsule space will move on to the next section of the renal tubule (proximal convoluted tubule PCT)
reabsorption
- ensures the body gets back small useful molecules from the filtrate
- eg amino acids , peptides , glucose , water , Na+ , Cl-
- substances to be reabsorbed pass through the tubular lumen , tubular wall and capillary wall back into the bloodstream
- 65% of reabsorption takes place in the PCT , and remaining in the DCT / LOH
- 80% of water , sodium chloride & bicarbonate are reabsorbed
- 100% of glucose & amino acids are reabsorbed [if they are detected in the urine , it could suggest problems]
sodium / glucose & amino acid / potassium & calcium / chloride / water reabsorption
sodium ;
- PCT , LOH , DCT
glucose & amino acid ;
- PCT
potassium & calcium ;
- PCT
chloride ;
- diffuses through in response to an imbalance created by sodium reabsorption
- moves with sodium
water ;
- follows ions through osmosis
- once sodium , glucose , amino acids & chloride have left tubular filtrate , water molecules follow
antidiuretic hormone (ADH) - urinary
- ADH from pituitary gland
- acts on DCT to promote water reabsorption
- tells DCT to reabsorb more water
- concentrates urine
- released when an animal is dehydrated or hypotensive to increase water content in the blood
alosterone
- from adrenal cortex
- acts on DCT to increase reabsorption of sodium
- tells DCT to reabsorb more sodium
- more sodium reabsorbed = osmotic imbalance = water molecules follow
- concentrates urine
- Na+ reabsorbed = K+ secrete
- if sodium is taken in, must be replaced by potassium to maintain salt balance
tubular secretion
- substances from blood capillaries move into tubules / urine
- substances not filtered in the glomerulus eg urea , ammonia OR due to reabsorption of sodium eg potassium , hydrogen for salt and acid-base balance
- occurs in PCT , LOH and DCT
renin - osmoregulation
- hormone secreted in glomerulus in response to low arterial pressure
- if pressure is low , glomerulus cannot filter blood to form glomerular filtrate
- converts angiotensinogen into angiotensin
angiotensin - osmoregulation
- vasoconstriction to raise blood pressure
- the constriction stimulates the release of aldosterone from adrenal cortex , causing water to be reabsorbed into the bloodstream
- more water = higher pressure = glomerulus able to filter blood to form glomerular filtrate
ureters
- leavers kidney at the hilus
- has 3 layers :
1. outer fibrous
2. middle smooth muscle (propels urine by peristaltic contractions)
3. inner layer lined with transitional epithelium (allows ureters to stretch as urine passes through) - enters urinary bladder at oblique angle , forming a valve to prevent back flow
- when bladder is full , urine presses on the entrance to close the valve
urinary bladder
- lined with transitional epithelium , can stretch as it is filled
- wall of the urinary bladder has smooth muscle
- around the neck of the urinary bladder , there are sphincter muscles
sphincter muscles
- internal sphincter : smooth muscles under involuntary control
- external sphincter : skeletal muscle under voluntary control , allows control of urination
- when the bladder is full , it sends a signal to the spinal cord which tells the smooth muscle / internal sphincter to tract
- the contraction of the smooth muscle when the bladder is full , we get the urge to pee
urethra
- continuation of the neck of urinary bladder
- carries urine from bladder to the external environment
- lined with transitional epithelium that allows it to stretch
- female ; shorter , straighter , only urinary function
- male ; longer , curved , runs down centre of the penis and also carries semen
control of urination process
- urination = excretion of urine from urinary bladder to urethra to external environment
- build-up of pressure in the bladder activates stretch receptors (senses pressure) in the bladder wall
- stretch receptors activate spinal reflex that cause smooth muscles of the bladder to contract
- contraction gives the sensation of needing to urinate
- voluntary control of the external sphincter (skeletal muscle) around the neck of the bladder allows temporary control of the urination
- house-trained animals exercise some form of voluntary control and temporarily hold urine (eg grass trained dogs)
- however , beyond a certain pressure limit , the sphincter must relax to release the urine
formation of urine
filtration -> reabsorption -> secretion -> concentration -> excretion
mitosis
- cell division that results in 2 daughter cells each having the same number and kind of chromosomes as the parent cell
- aka diploids
- daughter cells are identical to each other and parent cell
- typical form for tissue growth , but NOT for sperm and ovum .
meiosis
- cell division that results in daughter cells each with half the number of chromosomes as the parent cell
- aka haploids
- daughter cells are not identical to the parent cell
- sex cells eg sperm and ovum are produced this way
- this ensures the fertilised egg from the union of sperm and ovum has the same number of chromosomes as a parent cell (half + half = one)
components of male reproductive system
- testes
- dust system (epididymis , vas deferens , urethra)
- external genetalia (penis , scrotum)
- accessory glands (prostate gland , bulbourethral gland that dogs do not have , vesicle glands in rats only)
testes
- produces sperm cells and testosterone
- “held” inside the scrotum , outside the abdomen as normal internal body temperature is too high for sperm development
- concentration of testosterone influences the development of sperm and other male secondary sexual characteristics (eg bigger body, having penis and testes)
testes microscopic
- seminiferous tubules ; coiled mass of tubules , produces sperm
- seminiferous tubules eventually combine / join to form epididymis (a long convoluted tubule)
- interstitial cells ; between the tubules , secretes testosterone
scrotum
- skin pouch that houses testes
- regulates temperature of testes
- maintains testes at 3 degrees celsius lower than normal body temperature to protect sperm’s viability
- 2 muscles attached to the scrotum : cremaster and dartos , pull scrotum close to the body when it is cold and relax when it is warm
epididymis
- highly convoluted duct , caudal to testes
- tail of epididymis continues as vas deferens (epididymis eventually converge to form vas deferens . sperm goes from epididymis to vas deferens to prostate , urethra and out)
- sperm that is formed in the seminiferous tubules will mature as they pass into the epididymis
- sperm is stored in the epididymis before they are propelled along vas deferens during ejaculation
vas deferens
- aka ductus deferens
- carries sperm quickly from epididymis to urethra during ejaculation
penis
- urethra surrounded by layers of erectile tissue , muscle and connective tissue
- erectile tissues have many holes where blood can enter to erect the penis , enabling entry to the vagina
- penis has rich blood supply with many nerve endings
- delivers semen (sperm + fluids) from the testes into the female reproductive tract during mating
- also expels urine from the bladder to the outside via urethra
os penis
- embedded in erectile tissue close to the tip is the os penis
- os penis allows the entry of the penis into the vagina before erection is fully complete
accessory glands
- semen = sperm + fluid
- fluid = secretions from various accessory glands
- alkaline fluid (includes electrolytes, etc) to protect the sperm by counteracting the acidity of the female reproductive tract
- vagina = acidic , good for female’s health but hard for sperm to survive . alkaline fluid counteracts that
- different species have different combinations of accessory glands
prostate gland
- present in all domestic animals
- particularly large in dogs
bulbourethral glands
- only in cats and a few other animals
seminal vesicle glands
- not present in dogs and cats
- rats have
components of female reproductive system
- ovary
- uterine tube
- uterus (uterine horns , uterine body)
- cervix
- vagina
- vulva
ovaries
- lies on each side of the dorsal part of the abdominal cavity (very close to kidneys)
- produces ova/eggs
- produces oestrogen during follicle development stage and preparing for mating
- products progesterone to prepare for and maintain pregnancy by creating a comfortable environment in uterine lining
- contains primary follicles which develop into mature follicles
- number of follicles in a female is fixed from birth
primary follicles
- within ovarian connective tissue
- contains an immature ovum surrounded by a single layer of follicular cells
- each follicle has only one egg cell
- can develop into a mature follicle
mature follicle
- contains small amount of fluid and an ovum
- each follicle has only one egg cell
oviduct
- aka uterine tube / fallopian tube
- small tubes that extend from the tips of uterine horns
- finger-like projections at the tip known as fimbriae which capture the ovum released from ovaries
- also a site for fertilisation (sperm -> vagina -> cervix -> uterus -> oviduct -> meets ovum)
- cilia inside the oviduct move the fertilised ova slowly through uterine horns towards the uterus
- fertilised = embeds in uterus = pregnant
- not fertilised = still pushed eventually
- collects ova as they are released from ovaries
- conveys ova from ovaries to the uterine horns
- provide correct environment for the survival of both ova and sperm to aid fertilisation
uterus
- Y-shaped (horns and body)
3 walls ;
1. endometrium
2. myometrium
3. perimetrium
- provides a new site where embryos can develop into a baby
- provides means for developing embryos to receive nutrients from the mother via placenta / umbilical cord
endometrium - uterus
- inner mucosal layer
- a lot of blood supply
- secretes mucous and other substances
- allows implantation of a fertilised egg
- this layer thickens during pregnancy to provide nutrition to the embryo and support the development of the placenta
- nutrients from mother’s blood vessels -> placenta -> embryo
myometrium - uterus
- middle layer , smooth muscle
- strong contractions during parturition
- assists in pushing the baby out
perimetrium - uterus
- outer visceral layer of peritoneum
- suspends uterus from the dorsal body wall
- fixes uterus in its position
cervix
- short , thick-walled muscular sphincter
- connects uterine body with vagina
- lumen / inside space = cervical canal
- it is normally tightly closed to prevent uterine infections , only dilating to allow sperm or foetus to pass
- during pregnancy , cervical canal is blocked by a mucous plug which ensures bacteria and foreign particles cannot go in , protecting the embryo from infections
vagina
- a tube that leads to the external opening
- receives the penis at breeding
- serves as birth canal at birth
vulva
- external part of the female reproductive system
- guarded by a pair of vertical labia
- lying between the labia is a knob of erectile tissue aka clitoris
uniparous species
- one mature ovum produced per cycle only
- rare to have more
- humans , horses , cows
multiparous species
- multiple ova produced per cycle
- big litters
- cats , dogs , sow
ovarian cycle
- starts with primary follicle with an immature ovum in the centre
- follicle stimulating hormone (FSH from pituitary gland) stimulates selected follicles to mature
- as follicle grows , fluid holes form and continue to “merge” and get larger (more fluid)
- when the follicle and ovum have reached the maximum size , it is considered fully mature .
- oestrogen will be produced by follicular cells to assist in stimulating the release of the ovum
- luteinising hormone from pituitary gland is released and ovulation occurs as a result
- the surface of a mature follicle will weaken and rupture , releasing the ovum along with fluid into the oviduct
- empty follicle fills with blood after the ovum is released
- after an ovum has left the follicle , the empty follicle develops into corpus luteum
- if there is pregnancy , the corpus luteum is maintained through an endocrine signal
- if no pregnancy , the corpus luteum regresses
- cycle ends when corpus luteum has regressed.
corpus luteum
- after an ovum has left the follicle , the empty follicle develops into corpus luteum
- the process is influenced by the stimulation of luteinising hormone
- produces progesterone
- if there is pregnancy , the corpus luteum is maintained through an endocrine signal
- if no pregnancy , the corpus luteum regresses
progesterone
- necessary to maintain pregnancy
- thickens uterine lining and prepares for the implantation of fertilised egg
- allows fertilised egg to grow comfortably
- lack of progesterone may cause an unfavourable condition for the egg and may result in miscarriage
4 stages of estrous cycle - bitches
- proestrus (approx 9 days)
- estrus (approx 9 days)
- metestrus / diestrus (if not pregnant , 90 days. if pregnant , 50-60 days , delivery at approx 63 days after ovulation)
- anestrus (5 months)
proestrus - bitches
- initial phase, approximately 9 days
- body prepares for potential mating and pregnancy
- females release pheromones to attract males , but mating does not take place yet
- increased oestrogen in the blood (relate to maturation of follicles and ovarian cycle)
- oestrogen helps the body to prepare
- swelling of vulva
- bloody vulval discharge
estrus - bitches
- when follicle is mature enough to release the ovum
- approximately 9 days
- females become receptive to mating
- may actively seek out males and exhibit behavioural changes eg restlessness , changes in appetite , and increased frequency of urination
- oestrogen declines and luteinising hormone reaches its peak level
- triggers ovulation (releasing ovum)
- if a female mates with a male , fertilisation can take place
- corpus luteum forms in place of the empty follicle
- regress if not pregnant
- maintain and secrete progesterone if pregnant
metestrus / diestrus - bitches
- approximately 60-90 days
- if fertilisation has occurred , corpus luteum maintains and produces progesterone
- if no fertilisation has occurred , corpus luteum regresses and the female will enter a non-pregnant diestrus phase where uterus returns to the normal state
anestrus - btiches
- several months
- lack of ovarian activity , reproductive system rests before the next cycle begins
- if not pregnant , progesterone level gradually declines
- when a new cycle is about to start , some primary follicles will begin to develop
fertilisation
- ovum + sperm = fertilisation = zygote
- sperm is propelled up the ductus deferens into the urethra , through the penis and into the female reproductive tract
- sperm “swim” from the vagina through the cervix into the uterus
- once a sperm penetrates the ovum , further sperms are prevented from entering due to a rapid chemical change that thickens the ovum’s surface
- zygote = diploid = ready for mitosis
pregnancy
- after fertilisation , zygote undergoes mitosis
- as it is dividing , it also moves along the oviduct
- once it reaches the uterine horn , it has many cells and a fluid cavity ; it is now known as a blastocyst
- blastocyst implants in the uterine horn and continues to divide
- endometrium and myometrium are thickened by progesterone = new blood vessels grow and mucous secretes = more nutrients and comfortable for blastocyst to maintain pregnancy
- increased progesterone
- physical changes eg weight gain , enlargement of abdomen and development of mammary glands
stage 1 of parturition
- can last up to 24 hours
- restless , nesting behaviour
- decreased body temperature
- uterine contractions
- cervix gradually dilates to allow the passage of puppies
stage 2 of parturition
- can last up to a few hours to a full day
- active delivery
- uterine contractions intensify and female dog must push to deliver each puppy
- puppies are enclosed in individual amniotic sacs which may rupture during delivery
- mother typically breaks the sac , cleans the puppies and stimulates their breathing
- interval between each puppy can vary , but it usually ranges between 10 minutes to an hour
stage 3 of parturition
- involves the delivery of the placenta
- placenta nourished and protected puppies during gestation
- female dogs may eat the placenta which is natural as it can provide nutrients and clean the whelping area
major endocrine glands in cats
- pituitary gland
- thyroid gland
- parathyroid gland
- pancreas
- ovaries
- testes
- adrenal glands
mechanisms controlling hormone secretion
- nerve impulses (eg adrenaline from adrenal gland in response to nerve impulses from sympathetic nervous system)
- stimulating or releasing hormone (eg secretion of thyroid hormone is controlled by thyroid stimulating hormone from pituitary gland)
- levels of certain chemicals in the blood (eg glucose high , pancreas releases insulin)
- negative feedback system (eg oestrogen secreted after the follicle has matured prevents further secretion of FSH from pituitary gland)
pituitary gland
- small , ventral to the hypothalamus
2 portions with different functions and structure :
- anterior pituitary (cranial portion) ; produces hormone
- posterior pituitary (caudal portion) ; stores and releases hormones produced in the hypothalamus , does not produce any hormone
hormones released by pituitary gland (anterior pituitary)
- growth hormone
- prolactin
- thyroid-stimulating hormone (TSH)
- adrenocorticotropic hormone (ACTH)
- follicle-stimulating hormone (FSH)
- luteinising hormone (LH)
- interstitial cell stimulating hormone
growth hormone (GH)
- aka somatotropin hormone
- promotes body growth in young animals
- helps to regulate metabolism of proteins , carbohydrates and lipids
prolactin
- triggers and maintains lactation (secretion of milk from mammary glands)
- if nipple is stimulated by nursing , prolactin will continue to be released
thyroid-stimulating hormone (TSH)
- stimulates the development of the thyroid gland
- stimulates the thyroid gland to produce thyroid hormone
adrenocorticotropic hormone (ACTH)
- stimulates the growth and development of the adrenal gland
- stimulates the release of hormones from the adrenal gland (eg cortisol)
- in sudden stress , hypothalamus is stimulated and ACTH is released very quickly (cortisol -> signs of nervousness eg heart beating fast)
follicle stimulating hormone (FSH)
- female : growth and development of ovarian follicles (source of ovum)
- also stimulates follicular cells to secrete oestrogen (more oestrogen = negative feedback = less FSH)
- male : acts on tubules of the testis to aid sperm production
luteinising hormone (LH)
- stimulated by the presence of oestrogen in the blood
- stimulates mature follicles to rupture and release their ovum
- signals the empty follicle to form yellow body / corpus luteum
interstitial cell stimulating hormone (ICSH)
- stimulates the interstitial cells in the testes to secrete testosterone when concentration is low in the body
hormones stored and released by the pituitary gland (posterior pituitary)
- does not produce hormones
- only stores and releases hormones produced by the hypothalamus
- antidiuretic hormone (ADH)
- oxytocin
antidiuretic hormone (ADH) - endocrine
- acts on kidney
- reabsorbs more water from the urine and returns it to the bloodstream (more concentrated urine)
- released when the receptor in the hypothalamus detects a change of osmotic pressure in the blood due to dehydration
oxytocin - endocrine
- acts on mammary glands during late pregnancy
- causes milk to be released in response to a neonates suckling
- at the end of gestation , oxytocin causes the contraction of the smooth muscle of the uterus which results in parturition of the foetuses
thyroid gland
- ventral to first few rings of the trachea
- triiodothyronine (T3)
- thyroxine (T4)
- they regulate metabolic rate of body cells and are essential for normal growth
- allows animal to generate heat , maintain a constant internal body temperature and maintain blood glucose
- if metabolic rate is too high , nutrients are used too fast , body produces a lot of heat
** if metabolic rate is too low , nutrients are used too slow and body weight will increase with just a bit of eating . body doesn’t produce enough heat - calcitonin
calcitonin
- regulates calcium level in the blood (together with parathyroid hormone)
- lowers calcium levels by inhibiting the rate of decalcification of bone and stimulating bone growth
- tells bone not to release calcium into the blood and absorb more calcium from the blood
hypothyroidism
- undersecretion of thyroid hormones (especially T3 and T4)
- stunted growth
- hair loss
- slow heart rate
- fat
- sluggish
hyperthyroidism
- oversecretion of thyroid hormones (especially T3 and T4)
- hyperactive
- aggressive
- increased appetite
- fast heart rate
parathyroid gland
- small nodules in , on or near the thyroid glands
- parathyroid hormone (PTH / parathormone)
parathyroid hormone (PTH)
- maintain blood calcium levels
- along with calcitonin
- calcitonin deposits calcium from the blood into the bone when blood calcium levels are too high
- parathyroid hormone reabsorbs calcium from the bone into the blood when blood calcium levels are too low
pancreas
- located in the curve of the duodenum
- small islets inside the endocrine component have cells that secrete 3 hormones
- beta cells : insulin
- alpha cells : glucagon
- delta cells : somatostatin
insulin
- produced by beta cells of the pancreas
- response to high blood glucose
- decreases blood glucose by increasing the uptake of glucose into the blood cells and storing excess glucose as glycogen in the liver
- lack of insulin = diabetes mellitus , unable to lower blood glucose