Term 2 Lecture 13: The reproductive system and spermatogenesis Flashcards

1
Q

Asexual or clonal reproduction

A

-Fission, single celled organisms split in two

  • Budding, as seen in hydra, new individuals are formed by budding. In hydra the buds detach and become independent. In other organisms such as sponges they remain attached
  • Fragmentation e.g. a worm cut in two becomes two worms
  • Parthogenesis (aka virgin birth) - some animals produce offspring via eggs but without fertilisation. Offspring may be haploid or diploid depending on the species and living conditions. Aphids, bees and wasps can do parthogenesis. Water fleas lay two kinds of egg one that needs fertilisation and one that does not.
    When there is stress in the environment fertilisation provides gene mixing for natural selection which is advantageous.
    Snails also lay eggs that can develop without fertilisation.
    Komodo dragons lay unfertilised eggs that develop into offspring in good conditions. When conditions are not so good they find a mate and lay fertilised eggs.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Sexual reproduction generates diversity

A

Due to genetic variation via fusion of gametes produced by meiosis.
Different reproductive strategies enhance evolutionary fitness in animals

Strategies:
- synchronise release of gametes
- increase chance of fertilisation
- allow competition to determine sexual selection
- determined by competition

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Fertilisation requires an internal or external aquatic medium

A

For sperm to be able to swim to the egg.
The egg has attractants/ pheremones to guide sperm to it - which is particularly important in external fertilisation e.g. frog and fish eggs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Risks of external fertilisation

A
  • predators
    -water disturbance by wind, tide or current

as there is a risk of loss of gametes more eggs must be produced

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

In some species of bee (internal)

A

When they mate the males gonads are ripped off blocking the reproductive tract so that other bees cannot mate with that female

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

A species of fruit fly (internal)

A

Produces only one sperm with a tail 3x the length of its body. Presumably it is coiled in the male, once inserted into the female it blocks her reproductive tract so that she cannot mate with other males.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

In dragonflies (internal)

A

The end of a dragonfly penis is spoon shaped to scoop out competing sperm from other males whilst they deposit their own

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Frogs mate by amplexus ( external)

A

When a male clings to a female he causes her to release eggs which he then fertilises

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Internal fertilisation

A

Gametes are far more likely to meet than in external fertilisation

3 modes:
oviparous - egg birth
viviparous - live birth
ovoviviparous - live birth from eggs hatched inside mother

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Oviparous: Spiders, reptiles and birds

A

Spiders, reptiles and birds - internal fertilisation then they lay eggs that develop and young hatch out.
Birds will sit on their eggs to incubate them.
Turtles bury their eggs in warm sand.

(See oviparous egg diagram)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Oviparious: Monotremes

A

Echidna and platypus lay fertilised eggs that develop and hatch however they then feed their young with milk from their milk glands after they hatch.

(See oviparous egg diagram)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Viviparous: placental mammals and marsupials

A

-embryo retained in the mother
- have a uterus which develops a placenta to nourish the embryo

Marsupials have a poorly developed uterus/placenta so their babies are born at a very rudimentary stage of development, when born the babies climb directly into their mothers marsupial pouch (Aka marsupium) and attach to a nipple to feed and develop - technically externally.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Ovoviviparous: some fish, lizards, amphibians and snakes

A

Fertilised eggs are retained in the body so the embryos develop inside the eggs and hatch inside the mothers body before being born.
Some baby sharks eat each other in the womb before birth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Mating systems

A

-Monogamy <10% of mammals
-Promiscuity
-Polyandry (rare) one female with many males e.g. mole rats
-Polygyny one male many females is seen in 80% of mammals and leads to intense male competition

Female choice leads to sexual dimorphism - difference in male size, colour etc. to attract a mate and increase mating success.

In some cases females are larger to be able to carry eggs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Hermaphroditism is a variation on sexual reproduction

A

two types:

Simultaneous hermaphroditism:
Both mature egg and sperm producing tissue is present in the same individual at the same time.
Occurs in slugs and snails.
Often they do not fertilise themselves and their eggs and sperm can develop at different times.
This basically means that any two individuals can mate

Sequential hermaphroditism:
Individual will be protogynous - first a female or
protoandrous - first a male
Occurs in various fish species e.g. clown fish

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Sex determination and differentiation

A

Ovum always has an X chromosome

When fertilised with a sperm carrying a Y chromosome:
Genetic sex: embryo is XY male
Gonad sex: sex determining region of y chromosome (SRY) brings about development of undifferentiated into testes. The testes secrete masculinising hormones including testosterone a potent androgen
Phenotypic sex: in presence of testicular hormones the undifferentiated reproductive tract and external genitalia develop along male lines

When fertilised with a sperm carrying an X chromosome:
Genetic sex: embryo is XX female
Gonad sex - No Y so no SRY and undifferentiated gonads automatically develop along female lines
Phenotypic sex: No masculinising hormones so undifferentiated reproductive tract and external genitalia develop along female lines

Sexual differentiation has 3 levels:
Genetic: due to X or Y chromosome in sperm cell
Gonadal: development of testes with Y or ovaries without Y
Phenotypic: anatomical sex - sometimes this can be untrue as seen in CAH and the Guevodoces

17
Q

Congenital Adrenal Hyperplasia (CAH)

A
  • an inherited enzyme problem in the biosynthesis of cortisol
  • a block in this chemical path leads to too much or not enough cortisol production and lack of oestrogen formation.
  • deficiency of 21-beta - hydroxylase leads to reduction of cortisol and build up of androgen steroid precursors from the adrenals ( including, ultimately, testosterone)
  • this testosterone masculinises the anatomy of a female
18
Q

The Guevodoces of the Dominican Republic

A
  • if you are a male and don’t have enough testosterone influence in the embryo you look more female
  • guevedoces men appear female until puberty when they developed male characteristics and genitalia. They had a deficiency in 5-alpha reductase which converts testosterone to its active metabolite DHT.
    Post puberty higher testosterone levels caused masculinising development of male anatomy - they were also fertile.

From this research a 5-alpha-reductase inhibitor drug was developed for men suffering from benign prostate hyperplasia which causes prostate enlargement and excessive need to urinate. Reducing testosterone activation controls these symptoms.

It was also observed that guevedoces men did not develop male pattern baldness so there is also ongoing research into the development of a drug with this effect.

19
Q

Sex determination: the fight to be male

A

-sexual differentiation occurs early
- bipotential gonad has an outer cortex and an inner medulla
- sexual determination depends on SRY gene presence/absence
- TDF gene activates additional genes -> testes
- Testes produce AMH, DST and testosterone

20
Q

SRY gene directs male development

A

SRY gene produces testis determining SRY protein which initiates the production of multiple genes that cause gonad medulla to differentiate into tests which has:

Ledig cells: which secrete testosterone to control development of Wolffian duct into accessory structures and development of external genitalia via DHT

Sertoli cells: secrete anti-Mullerian hormone which controls the regression of the Mullerian duct

21
Q

Human reproductive anatomy consists of

A

Gonads: testes in males and ovaries in females. These produce the gametes.
Accessory sex glands: produce supporting secretions into the tract

Gamete production is spermatogenesis in males and oogenesis in females.

Gonads also produce sex hormones for secondary sexual characteristics as well as bone mineral density etc.

22
Q

Male reproductive system

A

See diagram - middle of notebook 3

Organs:
-Testes
- Accessory glands: seminal vesicles, prostate, bulbourethral glands
- penis
- Male tract: Epididymis, vas deferens and ureter

Risk of testicular cancer is greatly increased if testes are retained in the body ( do not descend during puberty)

23
Q

Gametogenesis: production of gametes

A

Sex steroidal hormones are derived from cholesterol: progesterone, cortisol, aldosterone and testosterone

production of gametes is under complex endocrine control

3 glands are involved in the production of hormones needed for gametogenesis:
-hypothalamus
- pituitary
- gonads
Together they are known as the Hypothalamopituitarygonadal axis

24
Q

Gametogenesis in men

A

Pulsatile release of GnRH from hypothalamus causes secretion of LH and FSH from pituitary which act on the testes to bring about production of testosterone and ultimately cause spermatogenesis

25
Q

Effects of testosterone

A

Acts on the reproductive system before birth
- masculinises tract, promotes descent of testes into scrotum

has an effect on sex-specific tissues after birth
-promotes growth/maturation of system at puberty
- needed for spermatogenesis and maintenance of tract as an adult

development of secondary sexual characteristics
- affects male pattern hair growth, deep voice, muscle growth

other reproductive related effects
- develops sex drive at puberty
- controls gonadotrophin hormone secretion

non- reproductive effects
-has a protein anabolic effect
-promotes bone growth and epiphyseal function
- causes aggressive behaviour

26
Q

Spermatogenesis

A

A complex process that generates a huge number of cells
that are highly specialised to travel a long distance.
closely associated with sertoli cells during development

sperm are formed of 3 parts:
Head - contains nucleus and at the tip an acromosomal vesicle carrying enzyme to digest its way into the ovum ( 5 micrometres)
Midpiece - filled with mitochondria to provide energy to the tail (5 micrometres)
Tail piece - capable of whip-like movement (50 micrometres)

27
Q

3 steps of spermatogenesis

A
  • mitotic division
  • meiotic division - to produce haploid cells
  • cytodifferentiation - to adapt the cells to the task - developing tail for movement

one spermatogonium splits into two spermatogonia
- one daughter cell stays at the outer edge of the seminepherous tubule to maintain the germ line
- the other daughter cell moves towards the lumen to produce spermatozoa

This daughter spermatogonium divides by mitosis twice to form 4 primary spermatocytes (46 chromosome diploid ds).
These primary spermatocytes divide by meiosis twice to give 16 spermatids (23 chromosome haploid ss).
These undergo cytodifferentiation to develop into fully formed spermatozoa (23 chromosome haploid ss)

Sperm is then stored and concentrated in the epididymis and ductus deferens.
During ejaculation it is mixed with secretions from the:
-seminal vesicles
-prostate gland
- bulbourethral glands

( see diagram of sertoli cell illustrating spermatogenesis middle of notebook 3)

28
Q

Release of sperm

A

sexual response cycle in males:
- excitement phase
- plateau phase
- orgasmic phase
- resolution phase

29
Q

Mechanism of erection

A

stimulation of mechanoreceptors in glans penis (tip)

  • parasympathetic/sympathetic supply to penile arterioles causes penile arterioles to dilate leading to erection that compresses the veins causing retention of blood and elongating the period of erection
  • parasympathetic supply to bulbourethral and urethral glands cause production of mucous to provide lubrication and aid the swimming of the sperm