Technology

Question 1

Image obtained with microscope relative to actual object

Answer 1

Upside down and backward

Question 2

Phase-contrast microscope

Answer 2

Light microscope. Enhances contrast, useful for living, unstained cells

Question 3

Transmission electron microscope

Answer 3

Useful for interiors. Tungsten filament in vacuum column is source of electrons. Drawbacks: tissue is dead, machine is delicate and expensive, specimens must be thin so only portions can be studied at a time.

Question 4

Scanning electron microscope

Answer 4

Surface, 3D, dead

Question 5

Ultracentrifuge

Answer 5

Isolates specific parts of cells in large quantities by cell fractionation. Tissue mashed in blender, then resulting liquid is spun in ultracentrifuge, separated by density.

Nuclei > mitochondria > ribosomes > clear liquid

Question 6

Freeze fracture/freeze etching

Answer 6

Cast of original tissue is made and examined