Techniques you should know

Question 1

single fluorophores “blink” by a process of random activation from an off or dark state, to an on or emission state, quickly followed by a switch back to a dark state or photobleaching. This process is sequentially repeated many times until most fluorophores have been imaged. For a successful imaging, individual fluorophores must be sparse enough, so that only one molecule is activated within a diffraction-limited region at any given time.

Answer 1

“Stochastic Optical Reconstruction Microscopy”

dSTORM