technique viva Flashcards by Dr Sumanth Devaraju | Brainscape

Q

how specimen reaches histopathology lab?

A

saline
formalin
transport media

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Q

onsite evaluation done by?

A

naked eye, hand lens and frozen

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Q

fresh biopsy

A

nerve - coz metachromatic granules disappear

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Q

muscle biopsy does not support paraffin sections

A

enzyme histochemistry can be done only

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Q

what are the reason for bad H&E section?

A

less than minimum 6 hours fixation

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Q

commercially available formalin

A

40% formaldehyde

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Q

Mechanism action

A

Methylene hydrate

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Q

washing formalin

A

50% removed by washing a week

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Q

formalin penetration

A

0.5mm/hour

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Q

Glutaraldehyde problem with PAS

A

false positive

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Q

ethanol

A

glycogen precipitated by this

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Q

picric acid explosive

A

when dry

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Q

can special stains be automated?

A

yes

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Q

What are the factor affecting tissue processing?

A

Heat
Agitation
viscosity
vacuum

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Q

types of microtome

A

rotary

base sledge

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Q

profile of blade

A

small profile

large profile

Q

angle of tilt?

A

10 to 20 degree

Q

how do you coat slides for histopathology?

A

egg albumin - mayers

Q

components of dyes?

A

chromophore - absorb light
auxochrome - side chain ionisable, reactive
sulphonic carboxylic phenolic

Q

types of auxochoromes

A

acidic
basic
neutral

Q

what is progressive staining?

A

dye allowed to act slowly

Q

What is regressive staining?

A

overstrain section and do controlled destain

Q

Color of hematoxylin

A

colourless

Q

active component of hematoxylin

A

hematin

Q

most often used counter stain for H?

A

eosin Y

Q

what is the effect of eosin on H?

A

makes nuclear blue to purple

Q

What is DPX?

A

Diphenyl burate phthalte