Technical Skills and Applications

Question 1

Decontamination

Answer 1

Reducing microbial levels on a surface so that is is safe to handle

Question 2

Disinfection

Answer 2

Reducing microbial levels, but does not kill spores Ex. 10% Lysol, 70% Ethanol, 10% Bleach

Question 3

Sterilization

Answer 3

100% Free of Microbes and spores Ex. Heat, Radiation, Gas

Question 4

Autoclaves

Answer 4

Steam Sterilize media and tips, very commonly used in labs

Question 5

pH meters measure _____________________ in solution

Answer 5

the concentration of hydrogen ion

Question 6

pH

Answer 6

-log[H+] - Typically first place probe in and read standard solutions pH 4/7/10 then sample

Question 7

Plasmids typically isolated after ______________, caught on ______________ then eluted with ___________, concentration measured by spectrophotometer @ ________ for DNA ( for protein)- __________- for purity

Answer 7

mini-prep, filter, water, 260, 280, 260:280

Question 8

Steps to creating monoclonal antibodies

Answer 8

1. Inoculate animal
2. Repeat inoculation
3. Harvest serum
4. Isolate single B cells
5. Fuse B cells with cancer cells, creating hybridoma

Question 9

What is a hybridoma?

Answer 9

B cell fused with a cancer cell