T1 Energy and respiration Flashcards

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1
Q

ATP function

A

transports chemical energy within the cell for use in metabolic processes

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2
Q

cellular respiration

A

set of metabolic reactions that convert biochemical energy into ATP

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3
Q

ATP

A

adenosine triphostphate

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4
Q

3 components of ATP

A
purine base (adenine)
pentose sugar (ribose)
3 * phosphate groups
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5
Q

where do the 3 phosphate groups in atp attach?

A

on the 5’ carbon of the pentose sugar

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6
Q

process by which energy is released from ATP

A

ATP hydrolysis of the terminal phosphate which is usually coupled to another cellular reaction to which energy is transferred

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7
Q

why does ATP release so much energy

A

the bonds between the phosphate groups contain electrons in a high energy state which store a large amount of energy
energy is released in formation of bonds through the hydrolysis reaction rather than breaking of bonds between phosphates.

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8
Q

end products of ATP hydrolysis

A

ADP and Pi

adenosine diphosphate and inorganic phosphate

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9
Q

mitochondrion membrane

A

double membrane
inner and outer membranes are separated by intermembrane space
inner membranes are folded for large reaction surface

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10
Q

where are amine oxidases found on the mitochondrion

A

on the outer membrane surface

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11
Q

where are phosphorylases found on the mitochondrion

A

between inner and outer membranes

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12
Q

cristae

A

inner membranes of mitochondrion

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13
Q

where are ATP synthases found on the mitochondrion

A

on the cristae

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14
Q

where are soluble enzymes for krebs cycle and fatty acid regulation found on the mitochondrion

A

in the matrix

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15
Q

matrix on the mitochondrion

A

space within the inner membrane

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16
Q

what processes are ATP used for

A

active transport (eg. phagocytosis)
cell division (mitosis)
binary fission in bacteria
temperature regulation

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17
Q

what catalyses the hydrolysis of the terminal phosphate on ATP

A

ATPase enzyme

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18
Q

ADP

A

low energy molecule that can be recharged by adding a phosphate

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19
Q

ATP hydrolysis process

A

water is split and added to the terminal phosphate to produce inorganic phosphate and ADP

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20
Q

energy released for every mole of ATP hydrolysed

A

30.7 KJ from the formation of chemical bonds

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21
Q

why are ATP phosphate bonds referred to as high energy bonds

A

as the covalent bonds contain electrons in a high energy state (therefore meaning bonds are relatively weak)
less energy required to break the bonds
when intermediates combine and form new bonds, much energy released

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22
Q

final product reactivity relative to reactants

A

final product is less reactive than reactants`

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23
Q

where does cellular respiration take place in the cell?

A

in the cytoplasm/mitochondria

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24
Q

word equation for cellular respiration

A

glucose + oxygen&raquo_space; carbon dioxide + water + ATP

25
Q

ATP production in cell stages

A

glycolysis
link reaction
Krebs cycle
electron transport and oxidative phosphorylation

26
Q

glycolysis

A

breakdown of glucose into 2 * pyruvate

27
Q

where does glycolysis take place

A

cytoplasm

28
Q

link reaction

A

when pyruvate is split and added to coenzyme A

29
Q

where does the link reaction happen

A

mitochondrial matrix

30
Q

krebs cycle

A

derivative of pyruvate decomposed to CO2

31
Q

where does the krebs cycle happen

A

mitochondrial matrix

32
Q

where does electron transport and oxidative phosphorylation take place

A

inner membranes of the mitochondrion (cristae)

33
Q

substrate level phosphorylation

A

enzyme transfers a phosphate group directly from a phosphorylated substrate to ATP

34
Q

oxidative phosphorylation`

A

glucose is oxidised in series of reduction and oxidation reactions providing energy for ATP formation

35
Q

respirometer

A

measures amount of oxygen consumed and amount of carbon dioxide produced during cellular respiration

36
Q

function of soda lime or potassium hydroxide in respiration rate measurement

A

absorbs carbon dioxide in respiration

37
Q

differential respirometers

A

use two chambers (control and a test chamber)

38
Q

respiratory quotient

A

ratio of the amount of carbon dioxide produced in cellular respiration to the amount of oxygen consumed in a set period.

39
Q

formula for respiratory quotient

A

(CO2 produced)/ (O2 consumed)

40
Q

carb RQ

A

1.0

41
Q

protein RQ

A

0.9

42
Q

fat RQ

A

0.7

43
Q

glucose oxidation type of reaction

A

catabolic, energy-yielding pathway

releases energy for ATP synthesis

44
Q

function of glycolysis and krebs cycle

A

supply electrons to the electron transport chain which drives oxidative phosphorylation

45
Q

glycolysis

A

glucose is broken down from a 6 carbon sugar into two molecules of pyruvate (pyruvic acid) (3 carbon acid)

46
Q

what is generated by glycolysis

A

2 ATP
2NADH
2H+

47
Q

glycolysis oxygen usage

A

anaerobic

48
Q

link reaction

A

pyruvate enters the mitochondrion and carbon dioxide is removed.
Coenzyme A picks up remaining 2 carbon fragment of pyruvate to form acetyl coenzyme A

49
Q

what happens when glucose is in short supply?

A

other organic molecules act as respiratory substrates

50
Q

examples of respiratory substrates

A

fats

proteins

51
Q

what are NAD and FAD

A

hydrogen acceptors, transporting hydrogens to electron transport chain

52
Q

what is NAD reduced to

A

NADH + H+

53
Q

what is FAD reduced to

A

FADH2

54
Q

krebs cycle

A

acetyl group passes into cyclic reaction and combines with a 4 carbon molecule to form a 6 carbon molecule, removing carbon as carbon dioxide ancd releasing coenzyme A for reuse

55
Q

electron transport chain

A

hydrogen pairs are transferred to the ETC
these are transferred along carriers in a series of redox reactions
releases energy used to phosphorylate ADPP to ATP
oxygen is final electron acceptor and is reduced to water

56
Q

what is the electron transport chain

A

a series of hydrogen and electron carriers on the membrane of the cristae

57
Q

ATP yield from krebs cycle

A

2

58
Q

ATP yield from ETC

A

34

59
Q

mitochondria size

A

0.5-1 micrometer