T1 Energy and respiration Flashcards

1
Q

ATP function

A

transports chemical energy within the cell for use in metabolic processes

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2
Q

cellular respiration

A

set of metabolic reactions that convert biochemical energy into ATP

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3
Q

ATP

A

adenosine triphostphate

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4
Q

3 components of ATP

A
purine base (adenine)
pentose sugar (ribose)
3 * phosphate groups
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5
Q

where do the 3 phosphate groups in atp attach?

A

on the 5’ carbon of the pentose sugar

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6
Q

process by which energy is released from ATP

A

ATP hydrolysis of the terminal phosphate which is usually coupled to another cellular reaction to which energy is transferred

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7
Q

why does ATP release so much energy

A

the bonds between the phosphate groups contain electrons in a high energy state which store a large amount of energy
energy is released in formation of bonds through the hydrolysis reaction rather than breaking of bonds between phosphates.

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8
Q

end products of ATP hydrolysis

A

ADP and Pi

adenosine diphosphate and inorganic phosphate

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9
Q

mitochondrion membrane

A

double membrane
inner and outer membranes are separated by intermembrane space
inner membranes are folded for large reaction surface

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10
Q

where are amine oxidases found on the mitochondrion

A

on the outer membrane surface

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11
Q

where are phosphorylases found on the mitochondrion

A

between inner and outer membranes

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12
Q

cristae

A

inner membranes of mitochondrion

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13
Q

where are ATP synthases found on the mitochondrion

A

on the cristae

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14
Q

where are soluble enzymes for krebs cycle and fatty acid regulation found on the mitochondrion

A

in the matrix

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15
Q

matrix on the mitochondrion

A

space within the inner membrane

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16
Q

what processes are ATP used for

A

active transport (eg. phagocytosis)
cell division (mitosis)
binary fission in bacteria
temperature regulation

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17
Q

what catalyses the hydrolysis of the terminal phosphate on ATP

A

ATPase enzyme

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18
Q

ADP

A

low energy molecule that can be recharged by adding a phosphate

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19
Q

ATP hydrolysis process

A

water is split and added to the terminal phosphate to produce inorganic phosphate and ADP

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20
Q

energy released for every mole of ATP hydrolysed

A

30.7 KJ from the formation of chemical bonds

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21
Q

why are ATP phosphate bonds referred to as high energy bonds

A

as the covalent bonds contain electrons in a high energy state (therefore meaning bonds are relatively weak)
less energy required to break the bonds
when intermediates combine and form new bonds, much energy released

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22
Q

final product reactivity relative to reactants

A

final product is less reactive than reactants`

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23
Q

where does cellular respiration take place in the cell?

A

in the cytoplasm/mitochondria

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24
Q

word equation for cellular respiration

A

glucose + oxygen&raquo_space; carbon dioxide + water + ATP

25
ATP production in cell stages
glycolysis link reaction Krebs cycle electron transport and oxidative phosphorylation
26
glycolysis
breakdown of glucose into 2 * pyruvate
27
where does glycolysis take place
cytoplasm
28
link reaction
when pyruvate is split and added to coenzyme A
29
where does the link reaction happen
mitochondrial matrix
30
krebs cycle
derivative of pyruvate decomposed to CO2
31
where does the krebs cycle happen
mitochondrial matrix
32
where does electron transport and oxidative phosphorylation take place
inner membranes of the mitochondrion (cristae)
33
substrate level phosphorylation
enzyme transfers a phosphate group directly from a phosphorylated substrate to ATP
34
oxidative phosphorylation`
glucose is oxidised in series of reduction and oxidation reactions providing energy for ATP formation
35
respirometer
measures amount of oxygen consumed and amount of carbon dioxide produced during cellular respiration
36
function of soda lime or potassium hydroxide in respiration rate measurement
absorbs carbon dioxide in respiration
37
differential respirometers
use two chambers (control and a test chamber)
38
respiratory quotient
ratio of the amount of carbon dioxide produced in cellular respiration to the amount of oxygen consumed in a set period.
39
formula for respiratory quotient
(CO2 produced)/ (O2 consumed)
40
carb RQ
1.0
41
protein RQ
0.9
42
fat RQ
0.7
43
glucose oxidation type of reaction
catabolic, energy-yielding pathway | releases energy for ATP synthesis
44
function of glycolysis and krebs cycle
supply electrons to the electron transport chain which drives oxidative phosphorylation
45
glycolysis
glucose is broken down from a 6 carbon sugar into two molecules of pyruvate (pyruvic acid) (3 carbon acid)
46
what is generated by glycolysis
2 ATP 2NADH 2H+
47
glycolysis oxygen usage
anaerobic
48
link reaction
pyruvate enters the mitochondrion and carbon dioxide is removed. Coenzyme A picks up remaining 2 carbon fragment of pyruvate to form acetyl coenzyme A
49
what happens when glucose is in short supply?
other organic molecules act as respiratory substrates
50
examples of respiratory substrates
fats | proteins
51
what are NAD and FAD
hydrogen acceptors, transporting hydrogens to electron transport chain
52
what is NAD reduced to
NADH + H+
53
what is FAD reduced to
FADH2
54
krebs cycle
acetyl group passes into cyclic reaction and combines with a 4 carbon molecule to form a 6 carbon molecule, removing carbon as carbon dioxide ancd releasing coenzyme A for reuse
55
electron transport chain
hydrogen pairs are transferred to the ETC these are transferred along carriers in a series of redox reactions releases energy used to phosphorylate ADPP to ATP oxygen is final electron acceptor and is reduced to water
56
what is the electron transport chain
a series of hydrogen and electron carriers on the membrane of the cristae
57
ATP yield from krebs cycle
2
58
ATP yield from ETC
34
59
mitochondria size
0.5-1 micrometer