T&H

Question 1

Cells must be in what phase of the cell cycle for cytogenic analysis

Answer 1

Mitosis

Question 2

Chromosomes are usually examined in what stage of mitosis

Answer 2

Metaphase

Question 3

How are banded chromosomes generated for analysis

Answer 3

Digestion with trypsin, followed by Giemsa stain

Question 4

Describe functions of topoisomerase I, DNA polymerase, RNA polymerase

Answer 4

Topoisomerase I - separates DNA strands during replication
DNA polymerase - adds nucleotides to 3’hydroxyl end of an RNA/DNA primer that is hybridized to a template, leading to synthesis of a new complementary strand of DNA
RNA polymerase - uses non-sense strand of DNA as a template to add bases to create mRNA in the sequence that is identical to the sense strand of DNA

Question 5

Coding regions of DNA are __ while noncoding are __

Answer 5

Exons, introns

Introns get spliced out during RNA maturation/before protein synthesis

Question 6

Southern blot, northern blot, western blot

Answer 6

Southern blot - used to analyze structure of DNA
Northern blot - RNA
Western blot - proteins

Question 7

In flow cytometry, forward scatter correlates with __ while side scatter correlates with __

Answer 7

Cell size ; granularity