Synthetic microbiology Flashcards
1
Q
What are the three steps to making artemisinic acid?
A
- Engineer farnesyl pyrophosphate pathway to overproduce FPP
- Induce amorphadiene synthetase gene from sweet wormwood to convert FPP to amorphadiene
- Cytochrome P450 converts amorphadiene to artemisinic acid
2
Q
How is bio-butanol made in E. coli?
A
2-keto acids are intermediates
Use ketoacid decarboxylases plus yeast alcohol dehydrogenase to produce butanol