Synthesis and Analysis Flashcards
How does mass spectrometry work?
Electrons in the spectrometer bombard the sample molecules and break electrons off, forming ions with different mass to charge ratios.
How do find the RMM of a compound from it’s mass spectrum?
Look at molecular ion peak (M peak), usually the one with the second highest mass charge ratio.
What is the M+1 peak and why is it there?
The peak 1 to the right of the M peak. Mostly due to the carbon-13 isotope which exists naturally and makes up about 1.1% of the carbon.
What can you use the M+1 peak for? What is the formula?
To find out how many carbon atoms there are in the molecule.
number of C atoms in organic compound= height of M+1 peak/height of M peak X 100
What is the M+2 peak and when does it occur?
If a molecule’s got either chlorine or bromine in it you get an M+2 peak.
It’s because chlorine and bromine have natural isotopes with different masses and they all show up on the spectrum.
Describe the peaks of compounds containing chlorine.
Chlorine’s two isotopes, Cl-35 and Cl-37, occur in the ratio of 3:1. So if a molecule contains chlorine, it will give an M peak and an M+2 peak with heights in the ratio 3:1
Describe the peaks of compounds containing Bromine.
Bromine’s got two isotopes, Br-79 and Br-81, that occur in equal amounts. So if a molecule contains bromine, the M peak and M+2 peak will both have the same height.
When would you see an M+4 peak?
When a molecule contains both halogens.
What is fragmentation?
In the mass spectrometer, the bombarding electrons make some of the molecular ions break up into fragments. The fragments that are ions show up on the mass spectrum , making a fragmentation pattern. Fragmentation patterns can be used to identify molecules and even their structure.
How do you work out the structural formula from mass spectra?
Got to work out what ion could have made each peal from its m/z value.
When is a fragment ion more abundant? What are two examples of stable fragment ions?
More stable.
Carbocations and acylium ions.
What is a carbocation?
An ion with a positively charged carbon.
Why are carbocations relatively stable?
Because alkyl groups feed electrons towards the +ve charge. You can show that an alkyl group is donating electrons by drawing an arrow on the bond that points to where the electrons are donated.
R–>–C+H2
What carbocation is the most stable?
Tertiary- 3 alkyl groups. More electrons donated to stabilise carbocation.
What is the acylium ion (RCO+) often formed from?
Aliphatic ketones.
What are the two forms of the acylium ion and what are they called?
RESONANCE FORMS
R-C+=O
R-C=O+ (triple C=O bond)
Actual structure somewhere in middle.
What does resonance in an ion do?
Helps stabilise what would otherwise be an unstable structure.
What are the two types of nuclear magnetic resonance (NMR) spectroscopy?
13C NMR- information about carbon atoms in a molecule are arranged.
1H(proton) NMR- How hydrogen atoms in a molecule are arranged.
When does an atomic nucleus have spin?
When it has an odd number of nucleons (protons and neutrons) in its nucleus. Causes it to have a weak magnetic spin.
What does NMR spectroscopy look at?
How the small magnetic field created by nuclear spin reacts when you put in a much larger external magnetic field.