Surface Plasom Resonance Flashcards
1
Q
What is measured?
A
The angle or reelection upon ligand binding to give information on binding
2
Q
What are the advantages of SPR?
A
Easy, quick, quantitative, label free, highly sensitive
3
Q
What are the disadvantages of SPR?
A
Requires expensive equipment, requires either the ligand or receptor to be bound.
4
Q
How is SPR set up?
A
A light source is set to shine through a prism which is sat on a thin metal layer, eg. Gold, which has the bound receptor on the underside of it, the ligand flow through a channel allowing binding to occur. Upon binding the angle at which the light reflects of the metal surface varies and is detected by a detector.
5
Q
What is SPR?
A
A quantitative surface sensitive optical detection methods.
