Supporting tissues Flashcards
Elastic cartilage (orcin)
* staining:
– hematoxylin – a basic dye, binds to negatively charged molecules, e.g.
DNA (in the nucleus)
– orcein: indicates the elastic fibers, membranes
* connective tissue types located in the section:
– elastic cartilage
– dense irregular CT
* connective tissue fibers located in the section:
– elastic fibers
– collagen fiber (orcein does not stain)
* connective tissue cells located in the section:
– Fix cells
* chondroblast
* chondrocyte
* fibrocyte
Hyaline cartilage HE
perichondrium
str. fibrosum
str. chondroblasticum
cartilage
interterritorial matrix
chondron (“territorium”) as histological unit
intraterritorial matrix
lacuna
chondrocyte
Fibrocartilage (HE)
interterritorial matrix
bundles of collagen fibers
chondron (“territorium”) as histological unit
intraterritorial matrix
lacuna
chondrocyte
bone
* staining: unstained
– Ground the inorganic substance is observable
* connective tissue types located in the section:
– lamellar (matured/ secondary) bone tissue
* connective tissue fibers located in the section:
– There are NOT fibers in the section
– remark: the lamellae contain spiral collagen fibers (as organic
element)
* connective tissue cells located in the section:
– There are NOT cells in the section
– remark: in the living bone the osteocytes are sitting in the
lacunae, and their processes are located in the canaliculi
Endochondral ossification HE
(1) 2 locations of bony development
① Primary ossification center
A. Bony development occurring in diaphysis
(A) Filled with red bone marrow
② Secondary ossification center
A. Bony development occurring in epiphysis (distal articular ends)
(A) As bone continue to elongate and increase in diameter
* ossification center→ cartilage is replaced
o But, cartilage : epiphyseal growth plate
(2) Occurs in 5 zones
① Resting Zone
A. chondrocytes are present in hyaline cartilage
② Proliferative Zone
A. chondrocytes divide & arrange parallely
③ Hypertrophic Zone
A. swollen chondrocytes & the glycogen in their cytoplasm are present
④ Calcification Zone
A. chondrocytes die & ECM calcifies here
⑤ Ossification Zone
A. lack of the chondrocytes
B. bone tissue presented