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MDSC 3312 MB > Superficial Fungi > Flashcards

Superficial Fungi Flashcards

1
Q

What is the compositions of SDA?

A

Non-inflammatory ‘black dot’ type

Inflammatory (‘ Kerion’ - rare )

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2
Q

Trichophyton rubrum culture

A

Trichophyton rubrum has a white cottony surface . Microconidia are piriform (pear-shaped) Macroconidia –Pencil shaped .

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3
Q

Epidermophyton rubrum

A

Colonies white , yellow coloured

slow growing & greenish-brown colored reverse pigment .

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4
Q

Epidermophyton floccusum fungal hyphae

A

macroconidia and fungal hyphae

Club shaped , smooth walled-septate.

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5
Q

Microsporum Canis

A

Colonies- flat , spreading, white to cream-coloured , with a dense cottony surface .
Have bright goldenyellow reverse pigment.

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6
Q

What is the length of treatment for skin and nail infections with dermatophytosis?

A

4 -6 weeks for - Skin infections.

Upto one year for - Nail infection.

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7
Q

What is the treatment method?

A

Removal of infected and dead epithelial structure & application of topical and oral antifungal

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8
Q

Oral antifugals

A

Griseofulvin – Oraly –Drug of choice.
Ketaconazole
Itraconazole
Terbinafine

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9
Q

What is the cause and presentation of shifting cloud?

A

Caused by :- Malassezia furfur / Pityrosporum orbiculare .

Disease is characterised by blotchy, hypopigmented or hyperpigmented macular lesion which may be associated with itching.

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10
Q

What is shifting cloud associated with and location?

A

associated with itching, Scattered over the chest , back , abdomen , neck & upper arm. = TINEA VERSICOLOR INFECTION = Malassezia furfur

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11
Q

Spaghetti and Meatball Appearance is associated with?

A

Lotta-Scattered over the chest , back , abdomen , neck & upper arm. = TINEA VERSICOLOR INFECTION = Malassezia furfur

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12
Q

What indicates Malassezia furfur on culture?

A

Fried egg colonies develops in 2 - 4 days at 37oc

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13
Q

What is the culture media for M furfur?

A

Skin scrapings from infected area inoculated in SDA -Sabouraud’s dextrose agar overlaid with film of olive oil

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14
Q

What does the smear from of M fufur look like?

A

Smear from culture shows bottle shaped budding yeast cells

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15
Q

Mal furfur is susceptible to?

A

ketoconazole

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16
Q

What is the treatment for a fungus that is light to dark grey on SDA? wHAT IS THE FUNGUS AND WHO IS IT COMMON IN?

A

Hortaea werneckii = Exophiala werneckii = Cladosporium werneckii common in females, micacanazole

17
Q

What does cladosporium werenckii

cause?

A

Superficial asymptomatic skin disease , characterised by light brown to black macular areas affecting thickly keratinised region of Palm & Soles .

18
Q

type of fungus of tinea nigra

A

dimorphic fungus

19
Q

What does the skin scraping for KOH SHOWS?

A

Skin scraping on KOH shows brownish branched septate hyphae & budding cells.

20
Q

What is Tricosporum beigelii characterized>

A

Skin scraping on KOH shows brownish branched septate hyphae & budding cells.

21
Q

Creamy white and urease positive is characteristic of?

A

t beigleii

22
Q

Black piedraie is associated with?Seen with

A

Black hard nodules on hair shafts of scalp & beard .

Black piedra mostly affects young adults & epidemics in families can occur due to sharing of combs.

23
Q

SDA colonies of black piedra

A

P.hortae produces reddish brown colonies when grown on SDA.P.hortae produces reddish brown colonies when grown on SDA.

24
Q

What is the treatment of black and white piedra?

A

Rx - Of - Black & White piedra
Remove hair & apply topical antifungal agents eg. Imidazoles
Amphotericin B lotion.

25
Q

What is the lab diagnosis method?

A

Nail ,
Hair clippings , Dermatophytes - Superficial
Skin scrapings .

Clean the part with 70% alcohol
Collect the material in a  Sterile Black  Paper  or  a Sterile Petri Dish to -
Allow drying of the specimen.
Reduce bacterial contamination.
Maintain viability.
26
Q

Direct Examination

A

Direct examination - 10 – 20 % KOH.
Fungal culture-Sabouraud’s dextrose agar (SDA).
Serological tests.
Skin tests.

PCR And Other Molecular Methods.

27
Q

What is used to investigate scalp lesions and what caution must be taken?

A

Scalp lesion – Wood lamp’s examination of infected hair – show fluorescence.

Stop anti fungals one week prior to collection.

28
Q

How can fungi be seen under the microscope and with what stain?

A

Scalp lesion – Wood lamp’s examination of infected hair – show fluorescence.

Stop anti fungals one week prior to collection.

29
Q

What is the composition of LPCB?

A

Lactic acid - preserves fungal structure.
Phenol – kills any live organism.
Glycerol – prevents drying.
Cotton blue – imparts blue color to structures.

MDSC 3312 MB (7 decks)

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