Summary of Technology Methods

Question 1

DNA gel electrophoresis

Answer 1

-presence, size, and quantity of purified DNA

Question 2

Souther Blot/ Assay

Answer 2

-presence and size of a specific DNA sequence in a complex mixture/sample

Question 3

PCR

Answer 3

-amplify a specific DNA sequence from a complex mixture

Question 4

rtPCR

Answer 4

-amplify a specific RNA sequence from a complex mixture

Question 5

multi-plex PCR

Answer 5

-amplify multiple specific sequences from a complex mixture in a single PCR reaction

Question 6

Norther Blot/Assay

Answer 6

-presence and size of a specific RNA sequence in a complex mixture/sample

Question 7

Denaturing gel eletrophoresis

Answer 7

-separate proteins by size/molecular weight

Question 8

Non-denaturing/Native protein gel

Answer 8

• separate proteins based on size, shape, and charge at a particular pH
• useful for mutations of one type of protein-hemoglobin

Question 9

Iso-electric focusing

Answer 9

-separate proteins based on pI (protein at which protein is uncharged

Question 10

Western Blot/Assay

Answer 10

-presence, size, and abundance of a specific protein in a complex mixture/sample

Question 11

Immuno-Fluorescence Microscopy

Answer 11

-the presence and localization of a protein in a fixed cell/tissue sample

Question 12

GFP Fluorescence

Answer 12

-determine the localization and dynamics of a protein of interest fused to GFP

Question 13

micro-assay based expression analysis

Answer 13

-determine the presence and relative abundance of all mRNA species in different samples/cells/tissues etc

Question 14

RNAi/morpholinos

Answer 14

-downregulate the expression of a specific gene in a cell/tissue

Question 15

morpholinos

Answer 15

• short, ss oligos complementary to a transcript you want to knock down
• inhibit expression by blocking progress of the ribosome/translation or by blocking a splice site and inhibiting splicing.

Question 16

RFLP

Answer 16

-restriction fragment length polymorphism