Sugar and water transport in Plants Lecture 9a Flashcards

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1
Q

Explain why plants loose water

A

Transpiration: loss of water via evaporation from the aerial parts of the plant.
• Transpiration occurs when 2 conditions are met:
1. Stomata are open.
2. Air surrounding the leaves is drier than the air inside the leaves.

–> If water that is lost is not replaced, cells will die.

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2
Q

Define transpiration.

A

loss of water via evaporation from the aerial

parts of the plant.

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3
Q

Define osmosis

A

Diffusion of water across a selectively permeable membrane from region of high water concentration (low solute concentration) to a region of low water concentration (high solute concentration)

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4
Q

Define water potential and state the direction in which water flows relative to water potential.

A

Water potential = the potential energy that water has in a particular environment, compared to the potential energy of pure water at room temperature under atmospheric pressure.
• Under these conditions, water potential = 0

Water always flows from areas of high water potential to areas of
low water potential.

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5
Q

Describe the two factors that affect water potential and how they do so.

A
  1. Solute concentration
    • Defined by the solution’s solute concentration relative to pure water.
    • The solute potential of pure water is 0.
    • Since solutes bind water molecules they lower the capacity of water to move and do work.
  2. Pressure
    is any kind of physical pressure on a solution.
    • Can be positive (push) or negative (tension) relative to atmospheric pressure. ex. turger pressure
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6
Q

State which way water will flow given the solute potential and pressure potential of two solutions.

A

Si = Si S + Si P

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7
Q

Explain how the physical properties of plant cells are changed when the plant is placed into solutions that have higher, lower, or the same solute concentration.

A

If the solution has higher solute concentration the water will move out of the cell (flacid)
if the solution has lower solute concentration water will move into the cell (turger pressure)
and if it is the same there will be no net movement

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8
Q

Define the terms flaccid, turgor pressure, and turgid

A

Flaccid- limp, droppy and soft
turgor pressure: The pressure exerted by water inside the cell against the cell wall.
Turgid: means distended or swollen, especially due to high fluid content. A plant cell is said to be turgid when its vacuoles have maximum amount of water.

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9
Q

Describe the role of turgor pressure in plant cells.

A

the cell turgor is the pressure of water on the walls of the cell, this pressure gives rigidity to the cell and thus to the plant and for that non woody plant can stand even though they have no wood and can resist bad environmental conditions.

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10
Q

Define bulk flow.

A

Movement of water due to a pressure gradient,
always occurs from higher to lower pressure and is independent of solute concentration
occurs in the trachieds and vessel elements of xylem and the seive- tube members of phloem

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11
Q

Differentiate between diffusion and bulk flow.

A

Diffusion is slow, Diffusion: Net movement down a
concentration gradient due to the random
motion of individual molecules. (Note:solutes may move independently of water.)

Bulk flow: Movement of water and solutes
together due to a pressure gradient

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12
Q

Relate the structure of sieve-tube members, vessel elements, and tracheids to their roles in bulk flow.

A

Sieve-tube members are cells that Must maintain sugar concentration so they are simplified for lower resistance

Vessel elements and tracheids are dead and empty for lower resistance

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13
Q

Explain the routes that soil solution can take from the soil to the vascular cylinder of the root.

A

transmembrane route: via water channels ( in and out of cells)

apoplastic route: within porus of cell walls (never moves into cells just goes through cell walls)

symplastic route: via plasmodemata (stays in cells)

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14
Q

Explain how the endodermis functions as a selective barrier between the root cortex and
vascular cylinder and why this function is important.

A

Endodermis = innermost layer of the cortex.
• Cylinder one cell thick.
• Forms a selective barrier between the cortex and the vascular cylinder.
• Within the walls of each cell is the Caspian strip.
• Waxy belt
• Forces the water and minerals passively moving through the apoplast to cross the plasma membrane of an endodermal cell before they can enter the vascular tissue!

important gate keeper to make sure bad things do not enter the plant

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15
Q

Explain the cohesion-tension hypothesis of how transpirational pull moves xylem sap up
from the roots to the leaves. Make sure to describe the importance of the cohesive and
adhesive properties of water and surface tension in your explanation.

A

The leading hypothesis to explain water movement in vascular plants is the cohesion-tension hypothesis.
• Water is pulled to the tops of trees via forces generated by transpiration at the leaf surface

The movement of fluid from root to shoot is driven by a water potential difference at opposite ends of xylem tissue.
• The water potential difference is created at the leaf end of the xylem by evaporation of water from leaf cells (i.e. transpiration).
• This evaporation lowers the water potential at the air-water interface, generating the negative pressure (i.e. tension).
• The negative pressure or pull is transmitted to the water in nearby cells
 water inside the xylem  water in the vascular tissue of roots  water in the soil.
• This continuous transmission of pulling forces is possible because the water molecules hydrogen bond to one another in a continuous fashion
(i.e., cohesion), makes it possible to pull xylem sap from above without water molecules separating.

adhesion!
• Cellulose is a polar molecule  strong attraction between water and the cellulose molecules in the xylem cell walls.
• This adhesion is believed to help offset the downward force of gravity.

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16
Q

Explain this statement: “The ascent of xylem sap is ultimately solar powered.”

A

The plant expends no energy in lifting the xylem sap.

17
Q

Define sugar source and sugar sink.

A

• Sugar source: a plant organ (especially mature leaves) in which sugar is produced by either photosynthesis or the breakdown of starch.
• Sugar sink: an organ (such as growing roots, buds, stems, and fruit) that is a net consumer or depository of sugar.
• A storage organ, such as a tuber or bulb
can be a source or a sink, depending on the season.

18
Q

Identify the organs of a plant that are sugar sources, sugar sinks, and those that can be
either.

A

Sinks: roots, Young leaves, flowers, fruits
sources: mature leaves

19
Q

Describe the processes of phloem loading and unloading and state whether each process
occurs by active transport or diffusion.

A

Loading: Sugar from mesophyll cells or other sources must be transported or loaded into sieve-tube elements before it can be exported to sugar sinks.

Sugar can move from mesophyll into the
companion cells and sieve tube via the symplastic route, or via a combination of symplastic and
apoplastic pathways.

Phloem cells can accumulate sucrose at higher [ ]s than what is found in the mesophyll cells  requires active transport to move sucrose against concentration gradient.

Unloading: Downstream, at the sink end of the sieve tube, phloem unloads its sucrose.
Do you think this unloading requires active transport, or is it accomplished via diffusion?
• It depends…
• Young growing parts of a plant - unloaded sugars are consumed during growth
• Concentration of free sugar in the sink is lower than the concentration in the sieve tube  diffusion
• Organs where the sucrose is converted to insoluble polymers like starch  diffusion
• Plants like sugar-beets that store sucrose within large vacuoles of root cells  active transport required to move sucrose into the vacuole, against its concentration gradient.

20
Q

Explain the mechanism by which phloem cells accumulate sucrose in the companion cells
and sieve-tube members at sugar sources (hint: think pump and cotransporter)

A
Phloem cells can accumulate sucrose 
at higher [ ]s than what is found in the 
mesophyll cells  requires active 
transport to move sucrose against 
concentration gradient.
21
Q

Define and describe the process of translocation. Trace the path of phloem sap from a
sugar source to a sugar sink and explain how this process is accomplished based on the
pressure flow hypothesis.

A
  • Pressure flow hypothesis
  • Driven by positive pressure.
  • Buildup of pressure at source end and reduction of pressure at sink end causes water to flow (along with sugars) from source to sink

High turgor pressure at source and low turgor pressure at sink, resulting from loading and unloading of sugars, drives phloem sap from source to sink via bulk flow.

22
Q

Compare and contrast the long distance transport of xylem sap to that of phloem sap .
Driving force
• How the pressure gradient is generated
• Direction of transport
• Structure of cells

A

Both xylem and phloem occur in plants

Phloem driven by positive pressure
pushing
potential is created as the sinks take up nutrients
Once and awhile active transport required to move sucrose into the vacuole, against its concentration gradient.

xylem driven by water potential differences
pulling
water potential created by transpiration
No energy involved

TO BE CONTINUED