Stuff to Memorize

Question 1

What’s the target of TLR-3?

Intra- or extracellular?

Answer 1

dsDNA

Intracellular

Question 2

What’s the target of TLR-7 and TLR-8

Intra- or extracellular?

Answer 2

ssRNA

Intracellular

Question 3

What’s the target of TLR-9?

Intra- or extracellular?

Answer 3

Demethylated CpG-rich DNA

Intracellular

Question 4

What’s the target of TLR-5

Intra- or extracellular?

Answer 4

Flagella

Extracellular

Question 5

What’s the target of dimerized TLR-1:TLR-2?

Intra- or extracellular?

Answer 5

Lipopeptides + GPI

Extracellular

Question 6

What’s the target of dimerized TLR-2:TLR-6?

Intra- or extracellular?

Answer 6

Lipoteichoic acid

Extracellular

Question 7

What’s the target of TLR-4?

Intra- or extracellular?

Answer 7

LPS

Extracellular

Question 8

What’s the target of TLR-2?

Intra- or extracellular?

Answer 8

Bacterial peptidoglycan

Extracellular

Question 9

Sources and actions of TNF

Answer 9

Macrophages, mast cells, T cells

Inflammation activation of endothelial cells (primarily).
Triggers expression of P and E selectins on endothelium to slow down neutrophils.
Neutrophil activation.

Question 10

Sources and actions of IL-1

Answer 10

Macrophages, DCs, endothelial cells, mast cells

Primarily acts on hypothalamus to trigger fever.
Also acts on BM to stimulate WBC production.
Triggers expression on P and E selectins on endothelium to slow down neutrophils.

Question 11

Sources and actions of IL-12

Answer 11

Macrophages, DCs

Increases IFN-γ production in NK and T cells.
Also triggers Th1 differentiation

Question 12

Sources and actions of IL-6

Answer 12

Macrophages, endothelial cells, T cells

Stimulates B cells to differentiate into plasma cells

Question 13

Sources and actions of IL-10

Answer 13

Macrophages, DCs, T cells

Leads to inhibition of cytokine production and decreased MHC II expression

Question 14

Sources and actions of IL-15

Answer 14

Macrophages

NK and T cell proliferation

Question 15

Sources and actions of IL-18

Answer 15

Macrophages

Acts on NK and T cells to increase production of IFN-γ (along with IL-12)

Question 16

Sources and actions of IFN-α and -β

Answer 16

α - DCs and macrophages
β - Fibroblasts

Increases MHC I expression, NK cell activation, and puts cells into antiviral state (activation of RNAase, inhibition of protein synthesis, etc.)

Question 17

Sources and actions of IFN-γ

Answer 17

NK and T cells

Activates macrophages

Question 18

Sources and actions of TGF-β

Answer 18

Macrophages

Inhibition of inflammation, and differentiation of Th17 + Treg cells

Question 19

Sources and actions of IL-7

Answer 19

Stromal cells of BM and thymus

First signal of lymphocyte production

Question 20

Sources and actions of IL-23

Answer 20

Macrophages + DCs

Increase T cell proliferation and Th17 differentiation

Question 21

CD for NK cells

Answer 21

16 and 56

Question 22

CD for mast cells

Answer 22

23

Question 23

CD for dendritic cells

Answer 23

80

Question 24

CD for monocytes

Answer 24

14

Question 25

CD for B cells

Answer 25

19 and 20

Question 26

CD for T helper cells

Answer 26

3 and 4

Question 27

CD for T cytotoxic cells

Answer 27

3 and 8

Question 28

CD for T regulatory cells

Answer 28

3, 4, and 25

Question 29

CD for neutrophils

Answer 29

15 and 16B

Question 30

CD for B 1 cells

Answer 30

5

Question 31

Cytokine secretion profile for Th1

Answer 31

IL-2, IFN-γ

Question 32

Cytokine secretion profile for Th2

Answer 32

IL-4/5/13

Question 33

Cytokine secretion profile for Th17

Answer 33

IL-17/22

Question 34

Cytokine secretion profile for T regulatory cells

Answer 34

TGF-β, IL-10