Study Unit 3 Flashcards
DNA Replication and Cell Division
What is cell division?
Cell division is the process by which cells make more cells, occurring for cell growth, replacement, healing, and reproduction.
What are the types of cell division in prokaryotes and eukaryotes?
Prokaryotes use binary fission, while eukaryotes undergo mitosis and meiosis.
Describe the genome differences between eukaryotes and prokaryotes.
Eukaryotes have a large, linear genome found in the nucleus, while prokaryotes have a small, circular genome located in the cytoplasm.
What are the two main phases of the eukaryotic cell cycle?
The two main phases are M phase (cell division) and interphase (preparation for division).
What are the stages of interphase in the eukaryotic cell cycle?
G1 phase (cell growth), S phase (DNA synthesis), G2 phase (preparation for mitosis), and G0 phase (cell not actively dividing).
What was the key finding of Meselson and Stahl’s experiment on DNA replication?
They confirmed that DNA replication is semiconservative, with each new DNA duplex consisting of one old strand and one new strand.
In what direction does DNA replication occur?
DNA replication occurs in the 5′ to 3′ direction.
What are Okazaki fragments?
Short segments of DNA synthesized on the lagging strand during DNA replication.
What role does DNA ligase play in DNA replication?
DNA ligase joins Okazaki fragments together by completing the sugar-phosphate backbone.
How do telomeres and telomerase function in eukaryotic cells?
Telomeres protect chromosome ends from shortening during replication, and telomerase extends these ends.
What are the main stages of mitosis?
Prophase, prometaphase, metaphase, anaphase, telophase.
What occurs during metaphase?
Chromosomes align along a single plane in the middle of the cell.
What is the role of cyclins and CDKs in cell cycle regulation?
Cyclins regulate the cell cycle by activating cyclin-dependent kinases (CDKs), which promote cell division.
What are the three major cell cycle checkpoints?
DNA damage checkpoint, DNA replication checkpoint, spindle assembly checkpoint.
What is apoptosis?
Apoptosis is programmed cell death, crucial for development and maintaining cellular health.
Define oncogenes and proto-oncogenes.
Oncogenes are cancer-causing genes, while proto-oncogenes are normal genes that can become oncogenes if mutated.