study questins Flashcards

1
Q

explain how a patient can test negative for RSV on the Sofia but positive on the Cephid

A

the cephid is more sensitive than the sofia. the sofia is an immunofluorescence assay for RSV antigen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

specimen and swab type for sofia Flu & RSV? media?

A

nasopharyngeal swab in 1 mL viral transport media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what should be done if the flu A & B were both positive on the Sofia?

A

invalid/indeterminate. notifying ordering physician and suggest ordering a 4-plex

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

if only one swab for rapid group A strep, what must be done prior to the assay being performed?

A

culture the swab first on SSA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what food can interfere with group A strep results?

A

cheese doritos

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what is the specimen swab type for the 4-plex &/or single covid testing? media?

A

nasopharyngeal swab in 3 mL viral transport media, universal media, or home brew

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what are the 4 viruses that are being tested for in the 4-plex?

A

RSV, Flu A, Flu B, covid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what secondary testing is performed in a positive C. diff from an inpatient sample? what does this secondary test tell the physician?

A

C. diff quick check. tells the provider if the patient has an active infection or if they are colonized with the bacteria (a carrier)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

when do we call inpatient positive MRSA>

A

always- get the patient into isolation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what specimen type is used for enterovirus testing?

A

CSF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what is the specimen collection type for RPA? media?

A

nasopharyngeal in 1-3 mL viral transport media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

specimen type for GIP? media?

A

stool in cairy-blair

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

how long do you have to inoculate infinity cartridges once opened?

A

30 mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

how long do you have to load the infinity cartridges once inoculated?

A

60 mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

which transport media will allow for N. gonorrhea to remain viable until the specimen can be cultured?

A

Eswab, amines with charcoal, anaerobic swab

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

a specimen arrives on a dry swab. can we do the culture?

A

no, a dry swab will not keep the organisms viable

17
Q

extra media is used to plate a CF culture if the specimen is received on an Eswab?

A

SSA

18
Q

an anaerobic culture on an abdominal wound is found in the fridge, is the specimen acceptable?

A

no, all anaerobic cultures need to be stored at room temp. Cold kills anaerobes

19
Q

media used for a stool culture?

A

CNA, HE, MAC, MAC with sorbitol, SS, GN broth

20
Q

chocolate agar contains which factors?

A

X & V

21
Q

what patient population is most likely to be infected with Burkholderia cepacia?

A

CF patients

22
Q

what media is used for a TB culture?

A

Lowenstein-Jensen slant, MGIT tube

23
Q

what is the best agar to use to isolate N. gonorrhea?

A

MTM

24
Q

list 3 media slants used to culture fungus and what temp/environment they incubate at

A

SAB-HI, inhibitory mold, mycobiotic slant @ room temp

25
Q

what culture site uses MRSA spectra agar & where is it incubated?

A

nasal swabs, incubated for 24 hours in non-CO2

26
Q

what media is used to culture yeast screens?

A

chromagar years

27
Q

what liquid nutrient is added to ARD body fluid cultures?

A

FOS

28
Q

reagents of gram stains & their purpose

A

crystal violet: stain the gram pos purple due to their thin cell well
iodine: make the crystal violet stay in the gram pos bacteria
decolorizer: remove crystal violet from gram neg bacteria
safranin: counter stain to stain the gram negs pink/red

29
Q

specimen rejection for respiratory cultures

A

> 10 epithelial cells / lpf

30
Q

specimen type used for yersenia culture, media & incubation

A

fresh stool in cairy-blair. MAC @ room temp

31
Q

how is a tissue specimen processed for aerobic culture?

A

TSB in tissue grinder, add specimen and homoginize
pipette onto media & streak it

32
Q

PCR assays

A

4plex
C. diff
Sars-CoV-2
MRSA
RPA
GIP
Factor II & V
Presurg MRSA
MTB-RIF
enterovirus

33
Q

what cultures get incubated in non-CO2

A

yeast, urine, stool, MRSA and Cipro

34
Q

what cultures get incubated at room temp?

A

fungus, yersenia, water