study guide Flashcards

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1
Q

why is learning about plant growth in drought conditions relevant to our lives

A

Since climate change is affecting us and will continue to affect us in the future, farmers will need to know how they can be the most productive in terms of their crop-yield. By observing what conditions plants grow best in, researchers can determine how to most efficiently produce crops.

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2
Q

Name the process by which plants obtain energy from the sun.

A

Photosynthesis

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3
Q
  1. What is the function of stomata? How might the stomata behave in dry conditions?
A

Stomata are the small pores on the underside of the leaf that regulate the flow of water, oxygen, and carbon dioxide in and out of the leaves. When it is closed, there is no gas exchange or water loss, and when it is open, there is oxygen and water loss, but carbon dioxide can enter for photosynthesis. Thus in dry conditions, the stomata are more likely to stay closed for longer periods of time in order to prevent water loss.

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4
Q

What do plants do with the energy that they have absorbed from the sun?

A

Plants capture the energy they have absorbed from the sun and convert it into usable energy (chemical energy in the form of glucose).

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5
Q

Where in a plant does photosynthesis take place?

A

Chloroplast

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6
Q

What is the main light absorbing green pigment in a plant?

A

Chlorophyll

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7
Q

What is the enzyme responsible for carbon fixation in a plant?

A

Rubisco

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8
Q

What is the overall research question of study 1 (tomato experiment)?

A

In a drought, how do shading conditions affect a Roma tomato plant’s growth, reproduction, and photosynthesis?

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9
Q

Given the description of an experiment, be able to identify its independent, dependent, and controlled variables.

A

The independent variables were shaded vs. unshaded conditions. The dependent variables are the growth traits, reproductive traits, and photosynthesis, which were the plant heights, number of leaflets, number of flowers, number of fruits, chlorophyll content, rate of photosynthesis, and average rubisco concentration. Drought conditions were the controlled variables.

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10
Q

Given the mean and the standard error of a data set, be able to calculate the 95% confidence interval.

A

We are 95% confident that the interval between 760 mm - 840 mm captures the true sample mean.

Equation:
Upper bound = m+2(SE)
Lower bound = m-2(SE)

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10
Q

What is the purpose of calculating the standard error (SE)/ standard error of the mean (SEM)?

A

The purpose of calculating standard error is to determine how close the sample mean is to the true population mean.

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11
Q

What is the purpose of a t-test?

A

The purpose of a t-test is to statistically compare the means of two groups. It is used to determine if the experiment/treatment had any effect on the groups.

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12
Q

Given the results of the t-test, be able to determine and explain whether there is a significant difference between the two groups. In our class, we will use a significance cutoff of 0.05 for the p-value.

A

The smaller the p value is below 0.05 the bigger the significant difference is between the two groups. However, when the P value is greater than 0.05 there will be no significant difference between the two groups.

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13
Q

What is the purpose of generating the light response curve in our tomato study?

A

The purpose of generating the light response curve in our tomato study is to be able to see how well a plant’s rate of photosynthesis depends on the light intensity. A major part is being able to see a plant’s maximum photosynthetic capacity through this light response curve. The CO2 intake was the variable being measured via the IRGA machine to see the plants photosynthesis rate.

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14
Q

What is the purpose of a standard curve?

A

The standard curve quantifies the relationship between protein concentration and light absorbance, to be able to calculate protein concentration (y=mx+b).

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15
Q

Given the equation of the standard curve and the absorbance value of a solution, be able to determine the protein concentration of that solution.

A

Example: standard curve equation: y = 2.09x + 1.02 Absorbance value = 3.99

Plug the absorbance value in for y, then solve for x
X = 1.42

16
Q

What is the purpose of the Bradford dye in the protein assay? How does the Bradford dye change with different concentrations of proteins in a solution?

A

The Bradford dye changes to a darker blue when more protein is present, as the solution gets darker more light is absorbed. This shows the protein concentration present in a certain solution.

16
Q

Why is it important to fully breakdown the leaf tissue in sodium hydroxide (NaOH) before using the leaf solutions in the protein assay?

A

Important that all rubisco is released

17
Q

In our lab, why do we need to generate a protein standard curve before we can determine the Rubisco concentration in a leaf?

A

We need to generate a protein standard curve first because we need to know how much protein is in the leaf. We can rely on the standard curve to convert absorbance into a protein concentration. The spectrophotometer only gives us the absorbance.

18
Q

In our lab, what percentage of the total protein in a leaf is assumed to be Rubisco?

A

½

19
Q

What is the common and scientific name of the animal that we will be studying in the second experimental module?

A

Common name -> Mealworms
Scientific name -> Tenebrio molitor

20
Q

Define “metabolic rate” of an animal.

A

Overall rate at which an organism’s body uses temperature/ expends energy

21
Q

List 5 factors that can impact the whole-animal metabolic rate.

A

Age
Gender
Body size
Physical activity
Diet

22
Q

Physiologists have found that the influence that temperature has on metabolism is “pervasive”. What does that mean?

A

It impacts all levels of functions in living organisms from molecules to physiological systems to the whole organismal level.

23
Q

Define these terms: endothermy, ectotherm, poikilothermy, homeothermy.

A

Endothermy: source of body temperature is reliant upon heat from metabolism.
Ectotherm: source of body temperature comes from the heat in the environment.
Poikilothermy: body temperature conforms to the environmental temperature.
Homeothermy: body temperature is regulated, constant, and independent of ambient body temperature.

24
Q

Which of the following terms best describe the mealworms? Endothermy, ectotherm, poikilothermy, homeothermy.

A

ectotherm

25
Q

In general, how would the metabolic rate change when a poikilotherm is acutely exposed to increasing temperature? Typically, for every 10 °C increase in temperature, how would the metabolic rate of a poikilotherm change?

A

The change is directly proportional to the changes in temperature. The increase for metabolic rate to every increase in this temperature is 2-3-fold. The metabolic rate increases exponentially with the body temperature increase.

26
Q

What is acclimation and acclimatization? How are they different from exposing animals to acute temperature changes?

A

Acclimation and acclimatization are terms that describe physiological adjustments when an animal is held long-term in a novel environment. They are different from exposing animals to acute temperature changes because acclimatization is the natural change in conditions, while acclimation is a controlled change in an animal’s environment.

27
Q

What is the method that we will use to measure the metabolic rate of the mealworms in our lab? What is the assumption behind using this method to determine the animal’s metabolic rate?

A

Respirometry: using the measurement of gas exchange of an animal to determine metabolic rate

Assumption: the main metabolic system that the organism is using is aerobic (aka oxidative metabolism), which means that O2 is the reactant and CO2 is the product