structure of the lac operon Flashcards
1
Q
what does the lac operon consist of?
A
- control elements
- 3 structural genes which code for enzymes that catalyse the breakdown & transport of lactose
2
Q
what are the control elements present in the lac operon?
A
- Catabolite Activator Protein (CAP) binding site
- promoter (lac P)
- operator (lac O)
3
Q
what is the function of the CAP binding site?
A
- it is the binding site of CAP
4
Q
what is the function of lac P?
A
- it is the binding site of RNA polymerase to transcribe structural genes
5
Q
what is the function of lac O?
A
- it is the binding site of the lac repressor protein, which is a product of the regulatory gene
6
Q
what are the 3 structural genes present in the lac operon?
A
- lac Z
- lac Y
- lac A
7
Q
what is the function of lac Z?
A
- it codes for β-galactosidase
8
Q
what is the function of β-galactosidase?
A
- it is an enzyme which hydrolyses lactose into glucose & galactose,
- & cleaves lactose into allolactose
9
Q
what is the function of lac Y?
A
- it codes for lac permease
10
Q
what is the function of lac permease?
A
- it is a membrane transport protein for the transport of lactose into the cells
11
Q
what is the function of lac A?
A
- it codes for β-galactoside transacetylase