Streptococcus pneumonia Metabolism and growth requirements Flashcards
What type of metabolism is streptococcus pneumonia?
Fermentative falcutative ( do not used oxygen in aerobe growth)
What are the growth requirements for streptococcus pneumonia?
Fastidious
reduced viability if allowed to dry at room temperature
What is used to identify Streptococuss pneumonia from other alpha hemolytic streptococci?
(1) optochin sensitivity
(2) bile solubility
(3) inulin fermentation
(4) quellung reaction
(5) the mouse virulence test.
What is the optochin sensitivity test?
Paper disk (P disk) have optochin placed on them and placed on a blood in inoculated with streptococcus pneumonia
What are the results of the optochin test if it is pneumococcus?
a zone of inhibition (no bacterial growth and no hemolysis) will be seen surrounding the optochin disk.
What is the bile solubility test?
Perform after bacteria grown up
What is the bile solubility test based on?
presence of an autolytic enzyme in the cell wall of pneumococci.
How is the enzyme activated in the bile solubility test?
bile and bile salts
What happens if there is pneumococci in the bile solubility test?
suspension will become clear, but if it is a viridans streptococcus it will remain turbid.
What is the inulin fermentation test?
plant polysaccharide is degraded and fermented with the production of acid by pneumococci, but not by other streptococci.
What is a positive in the queling test?
reaction between a pneumococcus and homologous antiserum, the capsule swells and becomes more refractile, making it easier to see.
What is the mouse virulence test?
intraperitoneal inoculation of mice with sputum from a patient with pneumonia.
Mouse dies and is cultured from.
can be detected in small numbers
