STREPTOCOCCI Flashcards
What is the GRAM CLASSIFICATION of Streptococci? Is it the same with Staphylococci?
Gram Positive, same with Staphylococci
What is the CATALASE RESULT of Streptococci? Is it the same with Staphylococci?
Catalase NEGATIVE. Staphylococci are catalase POSITIVE.
What is the OXIDASE RESULT of Streptococci? Is it the same with Staphylococci?
Oxidase NEGATIVE, same with Staphylococci.
Describe Streptococci in terms of KINGDOM, ORDER, FAMILY, GRAM CLASSIFICATION, CATALASE RESULT, and OXIDASE RESULT
Kingdom: Prokaryote/Monera
Order: Lactobacillales
Family: Streptococcaceae
Gram: POSITIVE
Catalase: NEGATIVE
Oxidase: NEGATIVE
What is the arrangement and morphology of Streptococci?
Chains/Clusters, Spherical
What are the three genera of Family Streptococcaceae?
Streptococcus
Enterococcus
Lactococcus
Previously known as Group D Streptococci
Enterococcus
Previously known as Group N Streptococci
Lactococcus
Describe Streptococci based on motility.
Non-motile
Streptococci are not difficult to kill because they don’t have spores. Therefore, Streptococci are described as?
Non-spore former
Size of Streptococci
0.5 to 2.0 um (micrometer)
Young cultures of Streptococci have ______
Capsules
Streptococci are (positive/negative) in gas production
Negative
Streptococci can/cannot ferment carbohydrates
Carbohydrate fermenters
Choices: BAP, NAP
Growth of Streptococci is more pronounced on ____ and poor on ____
Pronounced: BAP
Poor: NAP
The growth of Streptococci is enhanced by blood, therefore, they are _____ and grow best in _____ medium
Fastidious, BAP
Describe the culture of Streptococci.
Grayish
Pinpoint
Translucent to Slightly Opaque
Mucoid
Small and somewhat transparent
What are the hemolytic patterns of Streptococci?
Alpha (partial)
Beta (complete)
Gamma (non-hemolytic)
Streptococci may grow with or without the presence of Oxygen, therefore, they are _____
Facultative ANAEROBE
Streptococci are FACULTATIVE ANAEROBE, except _________.
Peptostreptococci
Describe Peptostreptococci
Obligate ANAEROBE, there must be NO oxygen for them to grow
Some species of Streptococci are _____ because they require increased concentration of CO2
Capnophilic
The cell wall of Streptococci is composed of thick layer of __________ and _________ layer
Peptidoglycan
Teichoic acid
Most Streptococci, except for most members of ____________, have a common ____________, a polysaccharide, on their cell walls that is used to classify Streptococci serologically. This classification scheme was developed by _______________ in 1930s (1933).
Viridans Group
Antigenic C Carbohydrate
Rebecca Lancefield
Classification scheme based on:
- Type and Patern of Hemolysis
- Physiologic divisions
- Antigenic properties
- Hemolytic pattern and temperature growth
- Brown’s Classification of Hemolysis
- Sherman’s Classification
- Lancefield Antigen Grouping
- Bergey’s Classification
BROWN’S CLASSIFICATION OF HEMOLYSIS
- Brown’s Classification is developed by _______ in 1919.
- The sources of blood are ____, ______, ______, _____.
- The most used blood is _______.
- What are the 4 classifications under this?
- J.H. Brown
- Sheep, Horse, Rabbit, Human
- Sheep Blood
*Traditional mixture: 5% sheep’s blood agar with trypticase soy agar
- Alpha, beta, gamma, alpha prime
BROWN’S CLASSIFICATION OF HEMOLYSIS
- Describe alpha hemolysis
- What are the two hemolysins that can cause alpha hemolysis?
- Alpha hemolysis is a reduction of _______
- What pigment color is produced around the colony?
- Incomplete/Partial hemolysis
- Streptolysin S and Streptolysin O
- Hemoglobin
- Green
BROWN’S CLASSIFICATION OF HEMOLYSIS
- Describe beta hemolysis
- In here, there is ______ area/zone around the bacterial growth
- Complete hemolysis of RBC
- Clear
BROWN’S CLASSIFICATION OF HEMOLYSIS
- Describe gamma hemolysis
- Is there a change in agar?
- Non-hemolytic/Lack of hemolysis
- None
BROWN’S CLASSIFICATION OF HEMOLYSIS
- Describe alpha prime hemolysis
- It is also known as ______.
- What specific hemolysis surrounds the colony?
- What specific hemolysis surrounds the hemolysis around the colony?
- Describe the zone of the two hemolysis
- Both alpha and beta hemolysis are present
- Wide zone
- Alpha hemolysis
- Beta hemolysis
- Small zone
SHERMAN’S CLASSIFICATION
- Sherman’s Classification is based on these 3 factors.
- What are the four physiologic divisions under this classification?
- Hemolytic reactions
Carbohydrate antigens
Phenotype tests - Pyogenic, Viridans, Lactococcus, Enterococcus
SHERMAN’S CLASSIFICATION
- ________ division includes the _______ hemolytic strains
- What are the strains in q1?
- Pyogenic, Beta
- A, B, C, D, E, F, G
SHERMAN’S CLASSIFICATION
- ______ classification includes Streptococci spp. that are not ____-tolerant
- This classification can’t grow at what range of temperature?
- Viridans, Salt
- 10-45°C
SHERMAN’S CLASSIFICATION
- This division is NOT clinically significant.
- This division is associated with what industry?
- Lactococci
- Dairy
LANCEFIELD’S CLASSIFICATION
- What are the first 5 groups?
- What are the clinically significant groups?
- Organisms are placed in ________ and are heated for ______ minutes to extract C Carbohydrates
- This carbohydrate forms the basis of serologic groupings (Groups A-H, and K-U)
- A, B, C, D, E
- A, B, C, D, E, F, G,
- Dilute acid, 10
- C Carbohydrate
BERGEY’S CLASSIFICATION (ACADEMIC)
What are the 4 classifications under Bergey’s Classification?
Pyogenic
Viridans
Enterococcus*
Lactococcus
BERGEY’S CLASSIFICATION (ACADEMIC)
Pyogenic
- They are mostly ___-hemolytic
- They neither grow on ____°C nor ____°C, they can only grow at ____°C
- Why do they only grow at the temperature in q2?
- Pyogenic produces _____ and _____
- Give 1 example
- Beta
- 10, 45, 37
- Because they mimic the body temp.
- Pus, Diseases
- Streptococcus pyogenes
BERGEY’S CLASSIFICATION (ACADEMIC)
Viridans
- They are mostly ___-hemolytic or ____-hemolytic
- Microbiota of __________________________
- Grow at ____°C and ____°C, but not at ____°C
- Give 1 example
- Alpha, Non
- Upper Respiratory Tract (URT)
- 37, 45, 10
- Enterococcus faecalis
BERGEY’S CLASSIFICATION (ACADEMIC)
Enterococcus*
- Microbiota of _______________
- Grow at _______°C temperatures
- Human intestines
- 10, 37, 45
BERGEY’S CLASSIFICATION (ACADEMIC)
Lactococcus
- Mostly _____-hemolytic with Group ____ antigens
- Found in _______ products
- Can grow at ___°C and ____°C
- Give 1 example
- This specie under Lactococcus causes the souring of milk
- Non, N
- Dairy
- 10, 37
- Streptococcus lactis
- Streptococcus lactis
Streptococcus pyogenes is under what Lancefield Classification?
Group A
Streptococcus pyogenes is also known as ___________ bacteria and ____________ bacteria
Fever-inducing, Flesh-eating
Streptococcus pyogenes is a normal/not normal flora
not a normal flora
Describe the colony of Streptococcus pyogenes
Smooth, glossy, round, mucoid
Why is the colony of S. pyogenes mucoid?
Because of the presence of M (emm) protein
The presence of __________ is an indicator of increased virulence of S. pyogenes
M (emm) protein (hairlike projections)
There are more than _____ types of M Proteins exist
> 200 (M1, M2…)
T/F. Cell wall structure of S. pyogenes is similar to that of other Streptococcus spp. and Gram+ bacteria
True
What are the three associated clinical manifestations with S. pyogenes
bacterial pharyngitis, skin infections, complications such as rheumatic heart disease and acute glomerulonephritis
Mode of transmission of S. pyogenes
Direct person to person
Indirect in aerosol droplets from coughing or sneezing
Streptococcus pyogenes
- Best defined virulence factor of S. pyogenes, encoded by _________
- Your answer in q1 contains ________
- Your answer in q1 is a surface protein found in _________ strains of S. pyogenes
- Function of your answer in q1
- M (emm) Protein, gene emm
- Hyaluronic acid
- Encapsulated
- Prevents phagocytosis to survive within the host
What are the 6 virulence factors of Streptococcus pyogenes
- Protein M (emm)
- Protein F (Fibronectin-binding protein)
- Lipoteichoic acid
- Hyaluronic Acid capsule
- Hemolysins
- Toxins and Enzymes
This virulence factor of S. pyogenes mediates epithelial cell attachment
Protein F (Fibronectin-binding protein)