Stock Pre-Work

Question 1

Amino Acid Composition

(acid hydrolysis)

Answer 1

• provides the composition of the protein, expressed as the percentage of each amino acid (i.e. 4.1% Ala, 3.8% Arg, 12.0% Asn/Asp, 3.4% Cys, etc.).

IDENTIFIES SMALL PEPTIDES

Question 2

N-terminal sequencing:

(Edman Degradation)

Answer 2

• provides sequence information for ~15-50 residues at the N-terminus of a protein
  
  • enough to unambiguously identify a protein

IDENTIFIES PROTEINS

Question 3

Mass spectrometry:

Answer 3

DETERMINES PRECISE MASS OF MOLECULES

Question 4

X-Ray Crystallography:

Answer 4

DETERMINES 3D STRUCTURES OF

LARGE PROTEINS AT ATOMIC RESOLUTION

Question 5

Nuclear Magnetic Resonance (NMR) Spectroscopy:

Answer 5

DETERMINES 3D STRUCTURES OF

SMALL PROTEINS AT ATOMIC RESOLUTION