sterilization methods Flashcards
whats the process of filteration
non-sterile solution pushed through a filter under pressure/ pulled through by vacuum into sterile receiving tank.
filter material must not shed particles or interact in any way.
pore size usually 0.2 mu example-HEPA
ISO class 5
what are applications of filteration
liquids and gases
heat liable drugs
heat sensitive injections
for treatment of air
what are advantages of filteration
absolulute sterilisation
for heat sensiyive media
removal of multiple particle sizes
what are disadvantages of filteration
each filter has a specific nominal pore size
unable to seperate organisms of the same size
whats the mode of action of moist heat autoclaving
moist heat destroys microorganisms by the irreversible denaturation of enzymes and structural proteins
requires precise control of time, temp and pressure
what are applications of moist heat
bioharzadoous trash
heat and mouisture resitant materials
sterilization of surgical dressing and medical devices
what are advantages of moist heat
nontoxic
easy to control and monitor
rapidly microbicidal
penetrates medical packing
what are disadvantages of moist heat
instruments can be damaged by repeated use- rust
can cause burns
whats the mode of action of ethylene oxide
alkylating agent
whats the applications of ETO
sterilisation of hermolabile substances like hormones and proteins
equipments such as catheters, syringes etc
what are advantages of ETO
suitable for heat and moisture sensitive materials
suitable for pre-packed products
what are disadvantages of ETO
slow
expensive
toxic substance produced
